Evaluation of different competitive immunoassays for aflatoxin M


Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
21 Feb 2019
Historique:
received: 10 04 2018
revised: 09 11 2018
accepted: 12 11 2018
entrez: 8 1 2019
pubmed: 8 1 2019
medline: 6 3 2019
Statut: ppublish

Résumé

Haptens (i.e. biomolecules which molecular weight is lower than 10 kDa) determination by inductively coupled plasma mass spectrometry (ICP-MS) is usually performed by means of competitive immunoassays. In these immunoassays, analyte quantification is indirectly carried out using two different tracer species (i.e. antibodies or antigen-protein conjugates). However, the benefits (and drawbacks) derived from using a given tracer species have not been systematically investigated so far. The goal of this work is to evaluate the influence of the tracer species employed in competitive immunoassays on the analytical figures of merit for aflatoxin M

Identifiants

pubmed: 30612640
pii: S0003-2670(18)31363-1
doi: 10.1016/j.aca.2018.11.024
pii:
doi:

Substances chimiques

Antibodies 0
Silver 3M4G523W1G
Aflatoxin M1 6795-23-9
Gold 7440-57-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

10-19

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Emma Pérez (E)

Department of Analytical Chemistry, Nutrition and Food Sciences, University of Alicante, PO Box 99, 03080, Alicante, Spain.

Francisco M Marco (FM)

Department of Biotechnology, University of Alicante, PO Box 99, 03080, Alicante, Spain.

Pascual Martínez-Peinado (P)

Department of Biotechnology, University of Alicante, PO Box 99, 03080, Alicante, Spain.

Juan Mora (J)

Department of Analytical Chemistry, Nutrition and Food Sciences, University of Alicante, PO Box 99, 03080, Alicante, Spain.

Guillermo Grindlay (G)

Department of Analytical Chemistry, Nutrition and Food Sciences, University of Alicante, PO Box 99, 03080, Alicante, Spain. Electronic address: guillermo.grindlay@ua.es.

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Classifications MeSH