Expression of complement C3, C5, C3aR and C5aR1 genes in resting and activated CD4
C3
C5
Complement expression
Intracellular complement
T-cells
Journal
Immunobiology
ISSN: 1878-3279
Titre abrégé: Immunobiology
Pays: Netherlands
ID NLM: 8002742
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
16
11
2018
revised:
21
12
2018
accepted:
21
12
2018
pubmed:
8
1
2019
medline:
10
1
2020
entrez:
8
1
2019
Statut:
ppublish
Résumé
Complement activation is traditionally thought to occur in the extracellular space. However, it has been suggested that complement proteins are activated and function at additional locations. T cells contain intracellular stores of C3 and C5 that can be cleaved into C3a and C5a and bind to intracellular receptors, which have been shown to be of vital importance for the differentiation and function of these cells. However, whether the origin of the complement proteins located within T cells is derived from endogenous produced complement or from an uptake dependent mechanism is unknown. The presence of intracellular C3 in T cells from normal donors was investigated by fluorescence microscopy and flow cytometry. Moreover, mRNA expression levels of several genes encoding for complement proteins with primary focus on C3, C3aR, C5 and C5aR1 during resting state and upon activation of CD4
Identifiants
pubmed: 30612786
pii: S0171-2985(18)30206-7
doi: 10.1016/j.imbio.2018.12.004
pii:
doi:
Substances chimiques
Biomarkers
0
C5AR1 protein, human
0
Complement C3
0
Complement C5
0
Receptor, Anaphylatoxin C5a
0
Receptors, Complement
0
complement C3a receptor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
307-315Informations de copyright
Copyright © 2018 Elsevier GmbH. All rights reserved.