Does low hydroxyl group surface density explain less bacterial adhesion on porous alumina?
Alloys
/ chemistry
Aluminum Oxide
/ chemistry
Bacterial Adhesion
Ceramics
/ chemistry
Humans
Joint Prosthesis
/ microbiology
Polyethylene
/ chemistry
Porosity
Prosthesis Design
Pseudomonas aeruginosa
/ physiology
Stainless Steel
/ chemistry
Staphylococcus aureus
/ physiology
Surface Properties
Titanium
/ chemistry
Adhesion
Alumina
Bacteria
Ceramic
Hydroxyl group
Journal
Orthopaedics & traumatology, surgery & research : OTSR
ISSN: 1877-0568
Titre abrégé: Orthop Traumatol Surg Res
Pays: France
ID NLM: 101494830
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
28
06
2018
revised:
13
11
2018
accepted:
28
11
2018
pubmed:
8
1
2019
medline:
23
2
2020
entrez:
8
1
2019
Statut:
ppublish
Résumé
Bacterial adhesion depends on surface materials. Recently it was suggested that ceramic-on-ceramic bearings could be less prone to infection than other bearings. We examined the possibility that porous alumina ceramic could be less susceptible to bacterial adhesion. As hydroxyl groups (OH) on material surface are a major factor governing the surface properties (for example: adsorption, first non-specific step of bacterial adhesion), we hypothesized that alumina had lower OH group density than other material. Thus, we asked (i) if bacterial adhesion was lower on alumina than on titanium alloy, stainless steel and polyethylene and (ii) if OH group density was also lower on alumina. We performed (i) in vitro bacterial cultures on porous alumina, titanium, stainless steel and polyethylene using Staphylococcus aureus and Pseudomonas aeruginosa, known to adhere to surfaces. Bacterial cultures were done 3 times in duplicate for each material and each strain. Colony Forming Units (CFU) per cm There was significantly less P. aeruginosa adherent on porous alumina (2.25×10 Bacterial adherence on porous alumina was lower than on other bearings. Although there were less surface OH groups on porous alumina, we failed establishing a statistical correlation between bacterial adherence and OH group density. IV, in vitro study.
Sections du résumé
BACKGROUND
Bacterial adhesion depends on surface materials. Recently it was suggested that ceramic-on-ceramic bearings could be less prone to infection than other bearings. We examined the possibility that porous alumina ceramic could be less susceptible to bacterial adhesion.
HYPOTHESIS
As hydroxyl groups (OH) on material surface are a major factor governing the surface properties (for example: adsorption, first non-specific step of bacterial adhesion), we hypothesized that alumina had lower OH group density than other material. Thus, we asked (i) if bacterial adhesion was lower on alumina than on titanium alloy, stainless steel and polyethylene and (ii) if OH group density was also lower on alumina.
MATERIAL AND METHODS
We performed (i) in vitro bacterial cultures on porous alumina, titanium, stainless steel and polyethylene using Staphylococcus aureus and Pseudomonas aeruginosa, known to adhere to surfaces. Bacterial cultures were done 3 times in duplicate for each material and each strain. Colony Forming Units (CFU) per cm
RESULTS
There was significantly less P. aeruginosa adherent on porous alumina (2.25×10
DISCUSSION
Bacterial adherence on porous alumina was lower than on other bearings. Although there were less surface OH groups on porous alumina, we failed establishing a statistical correlation between bacterial adherence and OH group density.
LEVEL OF EVIDENCE
IV, in vitro study.
Identifiants
pubmed: 30612953
pii: S1877-0568(18)30387-6
doi: 10.1016/j.otsr.2018.11.018
pii:
doi:
Substances chimiques
Alloys
0
Stainless Steel
12597-68-1
Polyethylene
9002-88-4
Titanium
D1JT611TNE
Aluminum Oxide
LMI26O6933
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
473-477Informations de copyright
Copyright © 2018 Elsevier Masson SAS. All rights reserved.