Dnmt1 is essential for egg production and embryo viability in the large milkweed bug, Oncopeltus fasciatus.

Animals DNA (Cytosine-5-)-Methyltransferase 1 / genetics DNA Methylation Embryonic Development Female Hemiptera / genetics Insect Proteins / genetics Male Oviparity
DNA methylation Dnmt1 RNAi Reproduction Transcription

Journal

Epigenetics & chromatin
ISSN: 1756-8935
Titre abrégé: Epigenetics Chromatin
Pays: England
ID NLM: 101471619

Informations de publication

Date de publication:
07 01 2019
Historique:
received: 20 10 2018
accepted: 18 12 2018
entrez: 9 1 2019
pubmed: 9 1 2019
medline: 7 3 2019
Statut: epublish

Résumé

The function of cytosine (DNA) methylation in insects remains inconclusive due to a lack of mutant and/or genetic studies. Here, we provide evidence for the functional role of the maintenance DNA methyltransferase 1 (Dnmt1) in an insect using experimental manipulation. Through RNA interference (RNAi), we successfully posttranscriptionally knocked down Dnmt1 in ovarian tissue of the hemipteran Oncopeltus fasciatus (the large milkweed bug). Individuals depleted for Dnmt1, and subsequently DNA methylation, failed to reproduce. Eggs were inviable and declined in number, and nuclei structure of follicular epithelium was aberrant. Erasure of DNA methylation from gene or transposon element bodies did not reveal a direct causal link to steady-state mRNA levels in somatic cells. These results reveal an important function of Dnmt1 seemingly not contingent on directly controlling gene expression. This study provides direct experimental evidence for a functional role of Dnmt1 in egg production and embryo viability and uncovers a trivial role, if any, for DNA methylation in control of gene expression in O. fasciatus.

Sections du résumé

BACKGROUND
The function of cytosine (DNA) methylation in insects remains inconclusive due to a lack of mutant and/or genetic studies.
RESULTS
Here, we provide evidence for the functional role of the maintenance DNA methyltransferase 1 (Dnmt1) in an insect using experimental manipulation. Through RNA interference (RNAi), we successfully posttranscriptionally knocked down Dnmt1 in ovarian tissue of the hemipteran Oncopeltus fasciatus (the large milkweed bug). Individuals depleted for Dnmt1, and subsequently DNA methylation, failed to reproduce. Eggs were inviable and declined in number, and nuclei structure of follicular epithelium was aberrant. Erasure of DNA methylation from gene or transposon element bodies did not reveal a direct causal link to steady-state mRNA levels in somatic cells. These results reveal an important function of Dnmt1 seemingly not contingent on directly controlling gene expression.
CONCLUSIONS
This study provides direct experimental evidence for a functional role of Dnmt1 in egg production and embryo viability and uncovers a trivial role, if any, for DNA methylation in control of gene expression in O. fasciatus.

Identifiants

DOI: 10.1186/s13072-018-0246-5 PMID: 30616649 PMC: PMC6322253
pubmed: 30616649
doi: 10.1186/s13072-018-0246-5
pii: 10.1186/s13072-018-0246-5
pmc: PMC6322253
doi:

Substances chimiques

Insect Proteins 0
DNA (Cytosine-5-)-Methyltransferase 1 EC 2.1.1.37

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

6

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Auteurs

Adam J Bewick (AJ)

Department of Genetics, University of Georgia, Athens, GA, 30602, USA.

Zachary Sanchez (Z)

Department of Entomology, University of Georgia, Athens, GA, 30602, USA.

Elizabeth C Mckinney (EC)

Department of Entomology, University of Georgia, Athens, GA, 30602, USA.

Allen J Moore (AJ)

Department of Entomology, University of Georgia, Athens, GA, 30602, USA.

Patricia J Moore (PJ)

Department of Entomology, University of Georgia, Athens, GA, 30602, USA. pjmoore@uga.edu.

Robert J Schmitz (RJ)

Department of Genetics, University of Georgia, Athens, GA, 30602, USA. schmitz@uga.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Dnmt1 is essential for egg production and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › Protéines liant le poly-adp-ribose › DNA (Cytosine-5-)-methyltransferase 1 › Dnmt1 is essential for egg production and...
questionsmedicales.fr › Phénomènes génétiques › Méthylation de l'ADN › Dnmt1 is essential for egg production and...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Développement embryonnaire et foetal › Développement embryonnaire › Dnmt1 is essential for egg production and...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Hemiptera › Dnmt1 is essential for egg production and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Dnmt1 is essential for egg production and...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Oviparité › Dnmt1 is essential for egg production and...