Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany.
Animals
Cat Diseases
/ epidemiology
Cats
Deer
/ microbiology
Dog Diseases
/ epidemiology
Dogs
Female
Germany
/ epidemiology
Gram-Negative Bacteria
/ immunology
Gram-Negative Bacterial Infections
/ microbiology
Humans
Male
Microbiota
Pets
Risk
Sequence Alignment
/ veterinary
Seroepidemiologic Studies
Tick-Borne Diseases
/ epidemiology
Ticks
/ microbiology
Bartonella
Dog
Illumina
Microbiome
Nanopore
One health
Roe deer
Tick
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
07 Jan 2019
07 Jan 2019
Historique:
received:
31
07
2018
accepted:
28
11
2018
entrez:
9
1
2019
pubmed:
9
1
2019
medline:
29
1
2019
Statut:
epublish
Résumé
Arthropod-borne diseases remain a major health-threat for humans and animals worldwide. To estimate the distribution of pathogenic agents and especially Bartonella spp., we conducted tick microbiome analysis and determination of the infection status of wild animals, pets and pet owners in the state of Hesse, Germany. In total, 189 engorged ticks collected from 163 animals were tested. Selected ticks were analyzed by next generation sequencing (NGS) and confirmatory PCRs, blood specimens of 48 wild animals were analyzed by PCR to confirm pathogen presence and sera of 54 dogs, one cat and 11 dog owners were analyzed by serology. Bartonella spp. were detected in 9.5% of all ticks and in the blood of 17 roe deer. Further data reveal the presence of the human and animal pathogenic species of genera in the family Spirochaetaceae (including Borrelia miyamotoi and Borrelia garinii), Bartonella spp. (mainly Bartonella schoenbuchensis), Rickettsia helvetica, Francisella tularensis and Anaplasma phagocytophilum in ticks. Co-infections with species of several genera were detected in nine ticks. One dog and five dog owners were seropositive for anti-Bartonella henselae-antibodies and one dog had antibodies against Rickettsia conorii. This study provides a snapshot of pathogens circulating in ticks in central Germany. A broad range of tick-borne pathogens are present in ticks, and especially in wild animals, with possible implications for animal and human health. However, a low incidence of Bartonella spp., especially Bartonella henselae, was detected. The high number of various detected pathogens suggests that ticks might serve as an excellent sentinel to detect and monitor zoonotic human pathogens.
Sections du résumé
BACKGROUND
BACKGROUND
Arthropod-borne diseases remain a major health-threat for humans and animals worldwide. To estimate the distribution of pathogenic agents and especially Bartonella spp., we conducted tick microbiome analysis and determination of the infection status of wild animals, pets and pet owners in the state of Hesse, Germany.
RESULTS
RESULTS
In total, 189 engorged ticks collected from 163 animals were tested. Selected ticks were analyzed by next generation sequencing (NGS) and confirmatory PCRs, blood specimens of 48 wild animals were analyzed by PCR to confirm pathogen presence and sera of 54 dogs, one cat and 11 dog owners were analyzed by serology. Bartonella spp. were detected in 9.5% of all ticks and in the blood of 17 roe deer. Further data reveal the presence of the human and animal pathogenic species of genera in the family Spirochaetaceae (including Borrelia miyamotoi and Borrelia garinii), Bartonella spp. (mainly Bartonella schoenbuchensis), Rickettsia helvetica, Francisella tularensis and Anaplasma phagocytophilum in ticks. Co-infections with species of several genera were detected in nine ticks. One dog and five dog owners were seropositive for anti-Bartonella henselae-antibodies and one dog had antibodies against Rickettsia conorii.
CONCLUSIONS
CONCLUSIONS
This study provides a snapshot of pathogens circulating in ticks in central Germany. A broad range of tick-borne pathogens are present in ticks, and especially in wild animals, with possible implications for animal and human health. However, a low incidence of Bartonella spp., especially Bartonella henselae, was detected. The high number of various detected pathogens suggests that ticks might serve as an excellent sentinel to detect and monitor zoonotic human pathogens.
Identifiants
pubmed: 30616666
doi: 10.1186/s13071-018-3240-7
pii: 10.1186/s13071-018-3240-7
pmc: PMC6322329
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11Subventions
Organisme : Robert Koch Institut, Berlin
ID : 1369-354
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB-TR84 TRR 84/2 2014
Organisme : BMBF CAPSyS
ID : 01ZX16004C
Organisme : Deutsches Zentrum für Infektionsforschung
ID : None
Organisme : Academy of Finland
ID : 295296, 2016-2020
Organisme : Sigrid Juséliuksen Säätiö
ID : Junior group leader grant, 2015-2018
Organisme : Forschungsgemeinschaft
ID : SFB-TR84 project B08 (TRR 84/3 2018)
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