Heteroexpression of Mycobacterium leprae hypothetical protein ML0190 provides protection against DNA-alkylating agent methyl methanesulfonate.
Alkylation
/ drug effects
Bacterial Proteins
/ genetics
DNA Damage
/ drug effects
Escherichia coli
/ drug effects
Genes, Bacterial
Genome, Bacterial
/ drug effects
Humans
Leprosy
/ microbiology
Methyl Methanesulfonate
/ toxicity
Models, Molecular
Mutagens
/ toxicity
Mycobacterium leprae
/ drug effects
Saccharomyces cerevisiae
/ drug effects
AlkB
Alkylation
DNA repair
Dioxygenase
ML0190
Mycobacterium leprae
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
12 02 2019
12 02 2019
Historique:
received:
11
12
2018
accepted:
30
12
2018
pubmed:
9
1
2019
medline:
5
11
2019
entrez:
9
1
2019
Statut:
ppublish
Résumé
Repair of DNA alkylation damage is essential for maintaining genome integrity and Fe(II)/2-oxoglutarate(2OG)-dependent dioxygenase family of enzymes play crucial role in repairing some of the alkylation damages. Alkylation repair protein-B (AlkB) of Escherichia coli belongs to Fe(II)/2OG-dependent dioxygenase family and carries out DNA dealkylation repair. We report here identification of a hypothetical Mycobacterium leprae protein (accession no. ML0190) from the genomic database and show that this 615-bp open reading frame encodes a protein with sequence and structural similarity to Fe(II)/2OG-dependent dioxygenase AlkB. We identified mRNA transcript of this gene in the M. leprae infected clinical skin biopsy samples isolated from the leprosy patients. Heterologous expression of ML0190 in methyl methane sulfonate (MMS) sensitive and DNA repair deficient strain of Saccharomyces cerevisiae and Escherichia coli resulted in resistance to alkylating agent MM. The results of the present study imply that Mycobacterium leprae ML0190 is involved in protecting the bacterial genome from DNA alkylation damage.
Identifiants
pubmed: 30616886
pii: S0006-291X(18)32871-7
doi: 10.1016/j.bbrc.2018.12.184
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Mutagens
0
Methyl Methanesulfonate
AT5C31J09G
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
779-783Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.