G418 induces programmed cell death in Acanthamoeba through the elevation of intracellular calcium and cytochrome c translocation.
Acanthamoeba
Amoebozoa
Apoptosis
Cytochrome c
G418
Programmed cell death
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
22
10
2018
accepted:
19
12
2018
pubmed:
9
1
2019
medline:
16
3
2019
entrez:
9
1
2019
Statut:
ppublish
Résumé
Acanthamoeba is a widely distributed opportunistic parasite which causes a vision-threatening keratitis and a life-threatening encephalitis. The cyst stage of this amoeba is especially resistant to currently used therapeutics and so alternative agents are urgently required. Growing evidence supports the existence of a programmed cell death system (PCD) in Acanthamoeba and while some features are shared by higher eukaryote cells, others differ. It is hoped that by understanding these differences we can exploit them as targets for novel drug intervention to activate PCD pathways in the amoebae but not the invaded human tissue. Here, we use the aminoglycoside G418 to activate PCD in Acanthamoeba. This drug caused a shape change in the treated amoebae. Cells rounded up and contracted, and after 6 h fragments of cells resembling the 'apoptotic bodies' of vertebrate cells were observed. G418 causes an increase in intracellular calcium from a resting level of 24 nM to 60 nM after 6 h of treatment. Mitochondrial function as assayed by the ΔΨ
Identifiants
pubmed: 30617503
doi: 10.1007/s00436-018-6192-0
pii: 10.1007/s00436-018-6192-0
pmc: PMC6349814
doi:
Substances chimiques
Amebicides
0
Gentamicins
0
Cytochromes c
9007-43-6
antibiotic G 418
A08F5XTI6G
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
641-651Références
Exp Parasitol. 2017 Dec;183:218-223
pubmed: 28916457
Parasit Vectors. 2010 Nov 09;3:104
pubmed: 21062457
Parasite. 2015;22:10
pubmed: 25687209
Exp Parasitol. 2014 Nov;145 Suppl:S39-45
pubmed: 24999094
Parasitol Res. 2018 Feb;117(2):571-577
pubmed: 29177581
Science. 1995 Feb 10;267(5199):836-7
pubmed: 7846528
Br J Cancer. 1972 Aug;26(4):239-57
pubmed: 4561027
J Biol Chem. 1962 Nov;237:3397-405
pubmed: 13933018
FASEB J. 2001 Mar;15(3):565-7
pubmed: 11259368
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
Cell Death Differ. 1996 Apr;3(2):229-36
pubmed: 17180087
Trends Parasitol. 2006 Nov;22(11):536-42
pubmed: 16942915
EMBO J. 1996 Oct 1;15(19):5177-83
pubmed: 8895562
Curr Biol. 1999 Sep 23;9(18):1061-4
pubmed: 10508616
Cell. 2010 Mar 5;140(5):631-42
pubmed: 20211133
J Eukaryot Microbiol. 1998 Jan-Feb;45(1):45-54
pubmed: 9495032
J Biol Chem. 2004 Jun 11;279(24):24911-4
pubmed: 15073175
Trends Parasitol. 2004 Aug;20(8):375-80
pubmed: 15246321
Acta Ophthalmol Scand. 2004 Apr;82(2):228-31
pubmed: 15043548
J Cell Biol. 1999 May 17;145(4):757-67
pubmed: 10330404
PLoS One. 2017 Aug 31;12(8):e0183795
pubmed: 28859105
Parasit Vectors. 2012 Jan 10;5:6
pubmed: 22229971
Int J Parasitol. 2002 Aug;32(9):1133-43
pubmed: 12117496
Parasitol Res. 2005 Mar;95(4):273-7
pubmed: 15678350
Exp Parasitol. 2018 Aug;191:31-35
pubmed: 29885293
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):12854-9
pubmed: 20615990
Org Biomol Chem. 2011 Jul 7;9(13):4766-9
pubmed: 21625690
Biochim Biophys Acta. 2005 Mar 22;1743(1-2):93-100
pubmed: 15777844
Parasitol Res. 2008 Apr;102(5):1069-72
pubmed: 18247052
Science. 1990 Dec 21;250(4988):1720-3
pubmed: 2125367
Parasitol Res. 2014 Sep;113(9):3151-8
pubmed: 24951165
J Cell Sci. 1994 Oct;107 ( Pt 10):2691-704
pubmed: 7876338
Acta Biochim Pol. 2007;54(4):797-803
pubmed: 18080020
Cell Death Differ. 2002 Jan;9(1):53-64
pubmed: 11803374
Antimicrob Agents Chemother. 2013 Jan;57(1):375-81
pubmed: 23114753
J Biol Chem. 1985 Mar 25;260(6):3440-50
pubmed: 3838314
Antimicrob Agents Chemother. 2017 May 24;61(6):
pubmed: 28320723
Curr Biol. 1998 Aug 27;8(17):955-8
pubmed: 9742396
Biol Direct. 2016 Dec 28;11(1):69
pubmed: 28031045
Hand Clin. 1985 Aug;1(3):383-90
pubmed: 3913671
J Basic Microbiol. 2017 Jul;57(7):574-579
pubmed: 28466971
Microbiology (Reading). 2007 Nov;153(Pt 11):3852-3863
pubmed: 17975094
Syst Biol. 2003 Oct;52(5):696-704
pubmed: 14530136
Exp Parasitol. 2017 Dec;183:85-91
pubmed: 29042273
Genome Biol. 2013 Feb 01;14(2):R11
pubmed: 23375108
Eur J Protistol. 2013 May;49(2):217-21
pubmed: 23290304
Microbiology (Reading). 2001 Sep;147(Pt 9):2409-2415
pubmed: 11535781
Nat Rev Mol Cell Biol. 2003 Jul;4(7):552-65
pubmed: 12838338
Antimicrob Agents Chemother. 2015 May;59(5):2817-24
pubmed: 25733513
Mol Biol Evol. 2010 Feb;27(2):221-4
pubmed: 19854763
J Clin Microbiol. 2002 May;40(5):1621-5
pubmed: 11980931