Cisterna Magna Injection in Rats to Study Glymphatic Function.
Cerebrospinal fluid
Cisterna magna injection
Glymphatic system
Tracer
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
9
1
2019
pubmed:
9
1
2019
medline:
10
7
2019
Statut:
ppublish
Résumé
The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.
Identifiants
pubmed: 30617975
doi: 10.1007/978-1-4939-9068-9_7
pmc: PMC6800144
mid: NIHMS1051910
doi:
Substances chimiques
Indicators and Reagents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
97-104Subventions
Organisme : NIA NIH HHS
ID : R01 AG048769
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG053991
Pays : United States
Organisme : NINDS NIH HHS
ID : RF1 NS110049
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS100366
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG057575
Pays : United States
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