Ultrasound-guided injection of the cranial tibial artery for stem cell administration in horses.
horse
ligament
regenerative medicine
scintigraphy
tendon
therapy
vascular
Journal
Equine veterinary journal
ISSN: 2042-3306
Titre abrégé: Equine Vet J
Pays: United States
ID NLM: 0173320
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
06
08
2018
accepted:
30
12
2018
pubmed:
10
1
2019
medline:
7
1
2020
entrez:
10
1
2019
Statut:
ppublish
Résumé
A technique for intra-arterial injection of mesenchymal stem cells (MSC) has been established for front limbs with the use of the median artery. This approach has been proposed for treatment of soft tissue injuries of the equine distal limb. A technique has not been validated yet for hindlimb injection. To assess the feasibility of injection of the cranial tibial artery in horses, and to evaluate the distribution and persistence of MSC after injection. In vivo experiment. In a first phase, the cranial tibial arteries of both hindlimbs of three research horses were catheterised with ultrasound guidance under general anaesthesia and injected with iodinated contrast. In the second phase, iodinated contrast was injected in three standing sedated horses with ultrasound guidance. In the final phase, Arterial injection was achieved in all 18 limbs without any significant complications. Mild partial periarterial injection was observed in four limbs. Scintigraphic images demonstrated diffuse MSC distribution from the tarsal area to the foot. Persistence decreased over time but signal was still present at 24 h. Limited retention of the radiolabel in the MSC. Ultrasound-guided injection of the cranial tibial artery can be performed both under general anaesthesia and standing sedation in horses. This technique could be used for MSC treatment of equine proximal suspensory desmopathy or other injuries in the distal hindlimb.
Sections du résumé
BACKGROUND
BACKGROUND
A technique for intra-arterial injection of mesenchymal stem cells (MSC) has been established for front limbs with the use of the median artery. This approach has been proposed for treatment of soft tissue injuries of the equine distal limb. A technique has not been validated yet for hindlimb injection.
OBJECTIVES
OBJECTIVE
To assess the feasibility of injection of the cranial tibial artery in horses, and to evaluate the distribution and persistence of MSC after injection.
STUDY DESIGN
METHODS
In vivo experiment.
METHODS
METHODS
In a first phase, the cranial tibial arteries of both hindlimbs of three research horses were catheterised with ultrasound guidance under general anaesthesia and injected with iodinated contrast. In the second phase, iodinated contrast was injected in three standing sedated horses with ultrasound guidance. In the final phase,
RESULTS
RESULTS
Arterial injection was achieved in all 18 limbs without any significant complications. Mild partial periarterial injection was observed in four limbs. Scintigraphic images demonstrated diffuse MSC distribution from the tarsal area to the foot. Persistence decreased over time but signal was still present at 24 h.
MAIN LIMITATIONS
CONCLUSIONS
Limited retention of the radiolabel in the MSC.
CONCLUSIONS
CONCLUSIONS
Ultrasound-guided injection of the cranial tibial artery can be performed both under general anaesthesia and standing sedation in horses. This technique could be used for MSC treatment of equine proximal suspensory desmopathy or other injuries in the distal hindlimb.
Substances chimiques
Contrast Media
0
Technetium Tc 99m Exametazime
3B744AG22N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
681-687Subventions
Organisme : UC Davis, Center for Equine Health
Informations de copyright
© 2019 EVJ Ltd.