A Mammalian Mitophagy Receptor, Bcl2-L-13, Recruits the ULK1 Complex to Induce Mitophagy.
Autophagy-Related Protein-1 Homolog
/ metabolism
Autophagy-Related Proteins
/ genetics
HEK293 Cells
Humans
Intracellular Signaling Peptides and Proteins
/ metabolism
Mitophagy
Protein Binding
Protein Kinases
/ genetics
Proto-Oncogene Proteins c-bcl-2
/ metabolism
Receptors, Cytoplasmic and Nuclear
/ genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
/ genetics
Atg32
Bcl2-L-13
mitochondria
mitophagy
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
08 01 2019
08 01 2019
Historique:
received:
05
07
2018
revised:
02
11
2018
accepted:
11
12
2018
entrez:
10
1
2019
pubmed:
10
1
2019
medline:
15
2
2020
Statut:
ppublish
Résumé
Degradation of mitochondria by selective autophagy, termed mitophagy, contributes to the control of mitochondrial quality. Bcl2-L-13 is a mammalian homolog of Atg32, which is an essential mitophagy receptor in yeast. However, the molecular machinery involved in Bcl2-L-13-mediated mitophagy remains to be elucidated. Here, we show that the ULK1 (unc-51-like kinase) complex is required for Bcl2-L-13 to process mitophagy. Screening of a series of yeast Atg mutants revealed that a different set of ATG genes is used for Bcl2-L-13- and Atg32-mediated mitophagy in yeast. The components of the Atg1 complex essential for starvation-induced autophagy were indispensable in Bcl2-L-13-, but not Atg32-mediated, mitophagy. The ULK1 complex, a counterpart of the Atg1 complex, is necessary for Bcl2-L-13-mediated mitophagy in mammalian cells. We propose a model where, upon mitophagy induction, Bcl2-L-13 recruits the ULK1 complex to process mitophagy and the interaction of LC3B with ULK1, as well as Bcl2-L-13, is important for the mitophagy.
Identifiants
pubmed: 30625316
pii: S2211-1247(18)31980-6
doi: 10.1016/j.celrep.2018.12.050
pmc: PMC6326162
pii:
doi:
Substances chimiques
Atg32 protein, S cerevisiae
0
Autophagy-Related Proteins
0
BCL2L13 protein, human
0
Intracellular Signaling Peptides and Proteins
0
Proto-Oncogene Proteins c-bcl-2
0
Receptors, Cytoplasmic and Nuclear
0
Saccharomyces cerevisiae Proteins
0
Protein Kinases
EC 2.7.-
ATG1 protein, S cerevisiae
EC 2.7.1.-
Autophagy-Related Protein-1 Homolog
EC 2.7.11.1
ULK1 protein, human
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
338-345.e6Subventions
Organisme : British Heart Foundation
ID : CH/11/3/29051
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RE/13/2/30182
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RG/16/15/32294
Pays : United Kingdom
Informations de copyright
Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
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