Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents.


Journal

Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252

Informations de publication

Date de publication:
02 2019
Historique:
received: 29 10 2018
revised: 06 12 2018
accepted: 04 01 2019
pubmed: 11 1 2019
medline: 19 12 2019
entrez: 11 1 2019
Statut: ppublish

Résumé

While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value.

Identifiants

pubmed: 30629949
pii: S0011-2240(18)30597-2
doi: 10.1016/j.cryobiol.2019.01.003
pii:
doi:

Substances chimiques

Cryoprotective Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

130-133

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Matthew J Powell-Palm (MJ)

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

Yanfang Zhang (Y)

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA; Department of Endocrinology and Metabolism, Luoyang Central Hospital Affliated to Zhengzhou University, Luoyang, 471009, PR China.

Justin Aruda (J)

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

Boris Rubinsky (B)

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA. Electronic address: rubinsky@berkeley.edu.

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Classifications MeSH