Proteome Analysis of Human Neutrophil Granulocytes From Patients With Monogenic Disease Using Data-independent Acquisition.
Diagnostic
Immunology*
Omics
Personalized medicine
Proteogenomics
data-independent acquisition
neutrophil granulocyte
primary immunodeficiency diseases
systems medicine
whole exome sequencing
Journal
Molecular & cellular proteomics : MCP
ISSN: 1535-9484
Titre abrégé: Mol Cell Proteomics
Pays: United States
ID NLM: 101125647
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
17
10
2018
revised:
14
12
2018
pubmed:
12
1
2019
medline:
16
1
2020
entrez:
12
1
2019
Statut:
ppublish
Résumé
Neutrophil granulocytes are critical mediators of innate immunity and tissue regeneration. Rare diseases of neutrophil granulocytes may affect their differentiation and/or functions. However, there are very few validated diagnostic tests assessing the functions of neutrophil granulocytes in these diseases. Here, we set out to probe omics analysis as a novel diagnostic platform for patients with defective differentiation and function of neutrophil granulocytes. We analyzed highly purified neutrophil granulocytes from 68 healthy individuals and 16 patients with rare monogenic diseases. Cells were isolated from fresh venous blood (purity >99%) and used to create a spectral library covering almost 8000 proteins using strong cation exchange fractionation. Patient neutrophil samples were then analyzed by data-independent acquisition proteomics, quantifying 4154 proteins in each sample. Neutrophils with mutations in the neutrophil elastase gene
Identifiants
pubmed: 30630937
pii: S1535-9476(20)31839-9
doi: 10.1074/mcp.RA118.001141
pmc: PMC6442368
pii:
doi:
Substances chimiques
Proteome
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
760-772Subventions
Organisme : Wellcome Trust
ID : 103139
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 103139/Z/13/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203149
Pays : United Kingdom
Informations de copyright
© 2019 Grabowski et al.
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