Membrane Hsp70-supported cell-to-cell connections via tunneling nanotubes revealed by live-cell STED nanoscopy.
Cell-to-cell connection
Cellular networks
Live-cell STED
Membrane-bound Hsp70
Tunneling nanotubes
Journal
Cell stress & chaperones
ISSN: 1466-1268
Titre abrégé: Cell Stress Chaperones
Pays: Netherlands
ID NLM: 9610925
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
16
10
2018
accepted:
03
12
2018
revised:
19
11
2018
pubmed:
12
1
2019
medline:
19
6
2019
entrez:
12
1
2019
Statut:
ppublish
Résumé
Heat shock protein Hsp70 (Hsp70) is found on the cell surface of a large variety of human and mouse tumor cell types including U87, GL261 glioblastoma, and 4T1 mammary carcinoma cells. We studied the role of membrane-bound Hsp70 (mHsp70) in the formation of cell-to-cell connections via tunneling nanotubes (TNTs) using live-cell STED nanoscopy. This technique allows the visualization of microstructures in the 100-nm range in the living cells. We could show that the presence of tumor-derived mHsp70 in TNTs with a diameter ranging from 120 to 140 nm predominantly originates from cholesterol-rich-microdomains containing the lipid compound globoyltriaosylceramide (Gb3). Under non-stress conditions, Hsp70 and Gb3 are structurally clustered in the membrane of TNTs of tumor cells that showed tumor type specific variations in the amount of cell-to-cell connection networks. Furthermore depletion of cholesterol and ionizing radiation as a stress factor results in a complete loss of Hsp70-containing TNTs.
Identifiants
pubmed: 30632067
doi: 10.1007/s12192-018-00958-w
pii: 10.1007/s12192-018-00958-w
pmc: PMC6363613
doi:
Substances chimiques
HSP70 Heat-Shock Proteins
0
Trihexosylceramides
0
globotriaosylceramide
71965-57-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
213-221Références
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