Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling.
coronavirus
human
infectious disease
microbiology
mouse
proximity labeling
replicase complex
translation
vesicular transport
virus
virus-host interaction
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 01 2019
11 01 2019
Historique:
received:
14
09
2018
accepted:
11
01
2019
pubmed:
12
1
2019
medline:
21
4
2020
entrez:
12
1
2019
Statut:
epublish
Résumé
Positive-sense RNA viruses hijack intracellular membranes that provide niches for viral RNA synthesis and a platform for interactions with host proteins. However, little is known about host factors at the interface between replicase complexes and the host cytoplasm. We engineered a biotin ligase into a coronaviral replication/transcription complex (RTC) and identified >500 host proteins constituting the RTC microenvironment. siRNA-silencing of each RTC-proximal host factor demonstrated importance of vesicular trafficking pathways, ubiquitin-dependent and autophagy-related processes, and translation initiation factors. Notably, detection of translation initiation factors at the RTC was instrumental to visualize and demonstrate active translation proximal to replication complexes of several coronaviruses. Collectively, we establish a spatial link between viral RNA synthesis and diverse host factors of unprecedented breadth. Our data may serve as a paradigm for other positive-strand RNA viruses and provide a starting point for a comprehensive analysis of critical virus-host interactions that represent targets for therapeutic intervention.
Identifiants
pubmed: 30632963
doi: 10.7554/eLife.42037
pii: 42037
pmc: PMC6372286
doi:
pii:
Substances chimiques
RNA, Viral
0
RNA-Dependent RNA Polymerase
EC 2.7.7.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : 173085
Pays : International
Organisme : European Commission
ID : 748627
Pays : International
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : 160780
Pays : International
Informations de copyright
© 2019, V'kovski et al.
Déclaration de conflit d'intérêts
PV, MG, JK, SP, NE, SB, CS, JP, HS, VG, RB, MS, MH, RD, VT No competing interests declared
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