Consequences of the Endogenous N-Glycosylation of Human Ribonuclease 1.

Asparagine / metabolism Enzyme Stability Glycosylation Humans Pichia / genetics Protein Denaturation RNA, Double-Stranded / metabolism Recombinant Proteins / chemistry Ribonuclease, Pancreatic / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Journal

Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623

Informations de publication

Date de publication:
19 02 2019
Historique:
pubmed: 12 1 2019
medline: 27 11 2019
entrez: 12 1 2019
Statut: ppublish

Résumé

Ribonuclease 1 (RNase 1) is the most prevalent human homologue of the archetypal enzyme RNase A. RNase 1 contains sequons for N-linked glycosylation at Asn34, Asn76, and Asn88 and is N-glycosylated at all three sites in vivo. The effect of N-glycosylation on the structure and function of RNase 1 is unknown. By using an engineered strain of the yeast Pichia pastoris, we installed a heptasaccharide (Man

Identifiants

DOI: 10.1021/acs.biochem.8b01246 PMID: 30633504 PMC: PMC6380942
pubmed: 30633504
doi: 10.1021/acs.biochem.8b01246
pmc: PMC6380942
mid: NIHMS1008659
doi:

Substances chimiques

RNA, Double-Stranded 0
Recombinant Proteins 0
Asparagine 7006-34-0
Ribonuclease, Pancreatic EC 3.1.27.5

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

987-996

Subventions

Organisme : NCI NIH HHS
ID : R01 CA073808
Pays : United States

Références

Methods Mol Biol. 2007;389:119-38
pubmed: 17951639
Protein Sci. 1992 Dec;1(12):1721-30
pubmed: 1304902
Biochem Soc Symp. 1974;(40):17-26
pubmed: 4620382
Mol Biotechnol. 2009 Sep;43(1):76-88
pubmed: 19507069
Biochemistry. 1997 Feb 25;36(8):2166-72
pubmed: 9047316
Biochem J. 2017 Jun 21;474(13):2219-2233
pubmed: 28495858
J Biol Chem. 1963 Apr;238:1396-401
pubmed: 13944116
Biochem J. 1951 Jul;49(2):215-8
pubmed: 14858313
J Biol Chem. 2014 Apr 4;289(14):9460-2
pubmed: 24554697
Biol Chem Hoppe Seyler. 1994 May;375(5):357-63
pubmed: 8074810
Science. 1939 Aug 4;90(2327):112-3
pubmed: 17810657
Methods Enzymol. 2001;341:81-94
pubmed: 11582813
J Biochem. 1986 Jan;99(1):17-25
pubmed: 3514589
Protein Expr Purif. 1996 May;7(3):253-61
pubmed: 8860650
Methods Enzymol. 2001;341:234-48
pubmed: 11582780
Eur J Biochem. 1993 Nov 15;218(1):239-44
pubmed: 8243469
Annu Rev Biochem. 2016 Jun 2;85:631-57
pubmed: 27294441
J Biol Chem. 1998 Dec 18;273(51):34134-8
pubmed: 9852072
J Biol Chem. 1953 Feb;200(2):493-506
pubmed: 13034806
Int J Mol Sci. 2016 Nov 25;17(12):
pubmed: 27898009
Carbohydr Res. 2009 Aug 17;344(12):1508-14
pubmed: 19608158
Biochemistry. 1998 Sep 1;37(35):12121-32
pubmed: 9724524
FEBS Lett. 2010 Jun 3;584(11):2194-200
pubmed: 20388512
Eur J Biochem. 1999 Jan;259(1-2):470-5
pubmed: 9914529
Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6062-7
pubmed: 15075390
Biochemistry. 2011 Sep 20;50(37):7835-41
pubmed: 21838247
Chem Rev. 1998 May 7;98(3):1045-1066
pubmed: 11848924
Cell Mol Life Sci. 1998 Aug;54(8):766-74
pubmed: 9760985
Biochem Biophys Res Commun. 2004 Mar 19;315(4):976-83
pubmed: 14985108
Anal Biochem. 1994 May 15;219(1):82-6
pubmed: 7520217
Glycobiology. 2004 Feb;14(2):103-14
pubmed: 14514716
Nat Protoc. 2009;4(1):58-70
pubmed: 19131957
J Mol Biol. 2007 Apr 27;368(2):434-49
pubmed: 17350650
Nat Protoc. 2007;2(9):2212-21
pubmed: 17853878
Glycobiology. 2007 Apr;17(4):388-400
pubmed: 17229815
J Biol Chem. 1968 Nov 25;243(22):5961-6
pubmed: 5696630
Trends Biochem Sci. 2010 Feb;35(2):74-82
pubmed: 19853458
FEBS Lett. 1979 Dec 15;108(2):341-4
pubmed: 520572
Science. 1972 Nov 3;178(4060):492-3
pubmed: 17754377
Biopolymers. 2008;90(3):259-77
pubmed: 17868092
J Pharm Sci. 2009 Apr;98(4):1223-45
pubmed: 18661536
Biochem J. 1988 Oct 15;255(2):501-5
pubmed: 3202829
Nucleic Acids Res. 1999 Sep 15;27(18):3696-701
pubmed: 10471739
Proteomics. 2014 May;14(10):1174-84
pubmed: 24687996
Science. 2012 Jun 1;336(6085):1150-3
pubmed: 22582013
J Protein Chem. 1998 Jul;17(5):397-405
pubmed: 9717736
Trends Biochem Sci. 2006 Mar;31(3):156-63
pubmed: 16473013
FEBS Lett. 1992 Aug 3;307(3):343-6
pubmed: 1322837
Glycobiology. 2003 Apr;13(4):227-44
pubmed: 12626415
Bioorg Med Chem. 1995 Dec;3(12):1565-78
pubmed: 8770382
J Biol Chem. 2014 Sep 19;289(38):25996-6006
pubmed: 25078100
Front Immunol. 2018 May 16;9:1012
pubmed: 29867984
FEBS Lett. 1993 Nov 1;333(3):233-7
pubmed: 7654266
Cancer Res. 1984 Apr;44(4):1682-7
pubmed: 6704974
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Biochemistry. 2016 Nov 22;55(46):6359-6362
pubmed: 27806571
Biochemistry. 1994 Jan 11;33(1):17-22
pubmed: 8286336
Mol Immunol. 2013 Oct;55(3-4):372-80
pubmed: 23597768
Eur J Biochem. 2000 Jan;267(2):566-72
pubmed: 10632727

Auteurs

Valerie T Ressler (VT)

Department of Chemistry , Massachusetts Institute of Technology , Cambridge , Massachusetts 02139 , United States.

Ronald T Raines (RT)

Department of Chemistry , Massachusetts Institute of Technology , Cambridge , Massachusetts 02139 , United States.
ORCID: 0000-0001-7164-1719

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Asparagine › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Glycosylation › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Pichia › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Dépliement des protéines › Dénaturation des protéines › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ARN double brin › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Ribonucléases › Endoribonucleases › Pancreatic ribonuclease › Consequences of the Endogenous N-Glycosylation...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Consequences of the Endogenous N-Glycosylation...