An ELISA using a recombinant chimera of protective antigen and lethal factor for serodiagnosis of cutaneous anthrax in India.
Animals
Anthrax
/ diagnosis
Antibodies, Bacterial
/ immunology
Antigens, Bacterial
/ immunology
Bacillus anthracis
/ immunology
Bacterial Toxins
/ immunology
Enzyme-Linked Immunosorbent Assay
/ methods
Humans
India
Recombinant Fusion Proteins
/ immunology
Reproducibility of Results
Sensitivity and Specificity
Serologic Tests
/ methods
Skin Diseases, Bacterial
/ diagnosis
Bacillus anthracis
Cutaneous anthrax
ELISA
Lethal factor
Protective antigen
Serodiagnosis
Journal
Biologicals : journal of the International Association of Biological Standardization
ISSN: 1095-8320
Titre abrégé: Biologicals
Pays: England
ID NLM: 9004494
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
05
03
2018
revised:
12
10
2018
accepted:
02
01
2019
pubmed:
13
1
2019
medline:
30
4
2019
entrez:
13
1
2019
Statut:
ppublish
Résumé
In this study, an ELISA was developed for simultaneous detection of antibodies against both the important toxins of B. anthracis i.e. protective antigen (PA) and lethal factor (LF). A chimera of PA and LF was made by fusion and cloned and expressed in E. coli. The purified recombinant protein was used in plate ELISA for serodiagnosis of anthrax. The chimera could detect antibodies against both the toxins of Bacillus anthracis. The human serum samples (n = 98) collected from anthrax endemic and non-endemic areas were tested employing ELISA. The ELISA gave sensitivity of 100% (95% Confidence Interval [CI], 92.13 to 100) and specificity of 97.78% (95% Confidence Interval [CI], 88.23 to 99.94) with a J index of 0.97. The efficiency of ELISA was found to be 98.9% with the positive predictive value (PPV) and negative predictive value (NPV) of 97.8% and 100%, respectively. The chimera of PA and LF could be a better diagnostic antigen for serodiagnosis as the assay detects antibodies against both the toxins in early as well delayed infection cases of anthrax. Therefore, it can be a very useful tool for the surveillance as well as for confirmation of cutaneous anthrax cases.
Identifiants
pubmed: 30635155
pii: S1045-1056(19)30001-6
doi: 10.1016/j.biologicals.2019.01.001
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Antigens, Bacterial
0
Bacterial Toxins
0
Recombinant Fusion Proteins
0
anthrax toxin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
55-60Informations de copyright
Copyright © 2019 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.