Cloning and characterization of a Flavin-free oxygen-insensitive azoreductase from Klebsiella oxytoca GS-4-08.
Azo Compounds
/ metabolism
Cloning, Molecular
Enzyme Stability
Escherichia coli
/ genetics
Gene Expression
Kinetics
Klebsiella oxytoca
/ enzymology
Molecular Weight
NADH, NADPH Oxidoreductases
/ chemistry
Nitroreductases
Oxidation-Reduction
Recombinant Proteins
/ chemistry
Substrate Specificity
Temperature
Azoreductase
Flavin
Klebsiella oxytoca
Oxygen-insensitive
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
03
09
2018
accepted:
09
01
2019
pubmed:
13
1
2019
medline:
4
6
2019
entrez:
13
1
2019
Statut:
ppublish
Résumé
This study reports the identification of a new bacterial azoreductase (AzoR) from Klebsiella oxytoca GS-4-08, its heterologous production in Escherichia coli and the decolorization of azo dyes. The AzoR, as a flavin-free, oxygen-insensitive enzyme, has a molecular mass of 22 kDa and a high substrate specificity for methyl red (MR). In the presence of 5 mM of NADH, the enzyme activity for decolorization of 50 mg l This study for the first time identified an AzoR from Klebsiella oxytoca strain, which enabled efficient degradation of azo dyes by strain GS-4-08.
Identifiants
pubmed: 30635809
doi: 10.1007/s10529-019-02647-9
pii: 10.1007/s10529-019-02647-9
doi:
Substances chimiques
Azo Compounds
0
Recombinant Proteins
0
NADH, NADPH Oxidoreductases
EC 1.6.-
Nitroreductases
EC 1.7.-
azoreductase
EC 1.7.1.17
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
371-378Subventions
Organisme : National Natural Science Foundation of China
ID : 51778298