Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies.
Journal
Nature nanotechnology
ISSN: 1748-3395
Titre abrégé: Nat Nanotechnol
Pays: England
ID NLM: 101283273
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
09
07
2018
accepted:
21
11
2018
pubmed:
16
1
2019
medline:
18
5
2019
entrez:
16
1
2019
Statut:
ppublish
Résumé
Although repetitive patterns of antigens are crucial for certain immune responses, an understanding of how antibodies bind and dynamically interact with various spatial arrangements of molecules is lacking. Hence, we introduced a new method in which molecularly precise nanoscale patterns of antigens are displayed using DNA origami and immobilized in a surface plasmon resonance set-up. Using antibodies with identical antigen-binding domains, we found that all the subclasses and isotypes studied bind bivalently to two antigens separated at distances that range from 3 to 17 nm. The binding affinities of these antibodies change with the antigen distances, with a distinct preference for antigens separated by approximately 16 nm, and considerable differences in spatial tolerance exist between IgM and IgG and between low- and high-affinity antibodies.
Identifiants
pubmed: 30643273
doi: 10.1038/s41565-018-0336-3
pii: 10.1038/s41565-018-0336-3
pmc: PMC6420075
mid: EMS80620
doi:
Substances chimiques
Antibodies
0
Antigens
0
Immunoglobulin G
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
184-190Commentaires et corrections
Type : CommentIn
Type : ErratumIn
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