Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles.
Adolescent
Adult
Aged
Alzheimer Disease
/ genetics
Biomarkers
Brain Concussion
/ genetics
Brain Injuries, Traumatic
/ diagnosis
CDC2 Protein Kinase
/ genetics
Casein Kinase Ialpha
/ genetics
Cathepsin D
/ genetics
Child
Emergency Service, Hospital
Extracellular Vesicles
/ genetics
Female
Gene Expression Regulation
/ genetics
Humans
Male
Middle Aged
Saliva
/ metabolism
Young Adult
biomarkers
extracellular vesicles (EVs)
real-time PCR
traumatic brain injury (TBI)
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
18
10
2018
accepted:
13
12
2018
pubmed:
16
1
2019
medline:
27
5
2020
entrez:
16
1
2019
Statut:
ppublish
Résumé
Traumatic brain injury (TBI) is a common cause of death and acquired disability in adults and children. Identifying biomarkers for mild TBI (mTBI) that can predict functional impairments on neuropsychiatric and neurocognitive testing after head trauma is yet to be firmly established. Extracellular vesicles (EVs) are known to traffic from the brain to the oral cavity and can be detected in saliva. We hypothesize the genetic profile of salivary EVs in patients who have suffered head trauma will differ from normal healthy controls, thus constituting a unique expression signature for mTBI. We enrolled a total of 54 subjects including for saliva sampling, 23 controls with no history of head traumas, 16 patients enrolled from an outpatient concussion clinic, and 15 patients from the emergency department who had sustained a head trauma within 24 hr. We performed real-time PCR of the salivary EVs of the 54 subjects profiling 96 genes from the TaqMan Human Alzheimer's disease array. Real-time PCR analysis revealed 57 (15 genes, p < 0.05) upregulated genes in emergency department patients and 56 (14 genes, p < 0.05) upregulated genes in concussion clinic patients when compared with controls. Three genes were upregulated in both the emergency department patients and concussion clinic patients: CDC2, CSNK1A1, and CTSD ( p < 0.05). Our results demonstrate that salivary EVs gene expression can serve as a viable source of biomarkers for mTBI. This study shows multiple Alzheimer's disease genes present after an mTBI.
Identifiants
pubmed: 30644102
doi: 10.1002/jcp.28139
pmc: PMC6478516
mid: NIHMS1005746
doi:
Substances chimiques
Biomarkers
0
CSNK1A1 protein, human
EC 2.7.11.1
Casein Kinase Ialpha
EC 2.7.11.1
CDC2 Protein Kinase
EC 2.7.11.22
CDK1 protein, human
EC 2.7.11.22
CTSD protein, human
EC 3.4.23.5
Cathepsin D
EC 3.4.23.5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
14377-14388Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM119943
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103468
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL116249
Pays : United States
Informations de copyright
© 2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.
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