Fine-mapping and validation of the genomic region underpinning pear red skin colour.


Journal

Horticulture research
ISSN: 2662-6810
Titre abrégé: Hortic Res
Pays: England
ID NLM: 101655540

Informations de publication

Date de publication:
2019
Historique:
received: 30 07 2018
revised: 08 11 2018
accepted: 15 11 2018
entrez: 18 1 2019
pubmed: 18 1 2019
medline: 18 1 2019
Statut: epublish

Résumé

Red skin colour is an important target trait in various pear breeding programmes. In this study, the genetic control of red skin colour was investigated in an interspecific population derived using the descendants of the red sport European pear cultivar 'Max Red Bartlett' (MRB) and the red-blushed Chinese pear cultivar 'Huobali'. Approximately 550 seedlings from nine families were phenotyped for red skin over-colour coverage (Ocolcov) and the intensity of red over-colour (Ocolint) on a 0-9 scale, and genotyped using genotyping-by-sequencing. Genome-wide association analyses were conducted using 7500 high-quality single nucleotide polymorphisms (SNPs). Genomic regions on linkage groups (LG) 4 and 5 were found to be associated, and the best SNP (S578_25116) on LG4 accounted for ~15% of phenotypic variation in Ocolcov and Ocolint. The association of S578_25116 with Ocolcov and Ocolint was successfully validated in a sample of ~200 European and Asian pear accessions. The association with red skin at locus S578_25116 was not present in Asian pear accessions, suggesting its close proximity to the MRB's Cardinal gene. Several putative candidate genes, including MYB transcription factors (

Identifiants

pubmed: 30651990
doi: 10.1038/s41438-018-0112-4
pii: 112
pmc: PMC6331550
doi:

Types de publication

Journal Article

Langues

eng

Pagination

29

Déclaration de conflit d'intérêts

The authors declare that they have no conflict of interest.

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Auteurs

Satish Kumar (S)

The New Zealand Institute for Plant and Food Research Limited, Hawkes Bay Research Centre, Havelock North, New Zealand.

Chris Kirk (C)

The New Zealand Institute for Plant and Food Research Limited, Palmerston North Research Centre, Palmerston North, New Zealand.

Cecilia Hong Deng (CH)

The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, Auckland, New Zealand.

Claudia Wiedow (C)

The New Zealand Institute for Plant and Food Research Limited, Palmerston North Research Centre, Palmerston North, New Zealand.

Mengfan Qin (M)

4Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095 Nanjing, China.

Richard Espley (R)

The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, Auckland, New Zealand.

Jun Wu (J)

4Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095 Nanjing, China.

Lester Brewer (L)

The New Zealand Institute for Plant and Food Research Limited, Motueka Research Centre, Motueka, New Zealand.

Classifications MeSH