Establishment of a New Scirrhous Gastric Cancer Cell Line with FGFR2 Overexpression, OCUM-14.
Adenocarcinoma, Scirrhous
/ drug therapy
Animals
Antineoplastic Agents
/ pharmacology
Cell Culture Techniques
Cell Proliferation
/ drug effects
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
Male
Mice, Nude
Middle Aged
Receptor, Fibroblast Growth Factor, Type 2
/ genetics
Stomach Neoplasms
/ drug therapy
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Journal
Annals of surgical oncology
ISSN: 1534-4681
Titre abrégé: Ann Surg Oncol
Pays: United States
ID NLM: 9420840
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
27
08
2018
pubmed:
18
1
2019
medline:
10
7
2019
entrez:
18
1
2019
Statut:
ppublish
Résumé
The prognosis of scirrhous gastric carcinoma (SGC), which is characterized by rapid infiltration and proliferation of cancer cells accompanied by extensive stromal fibrosis, is extremely poor. In this study, we report the establishment of a unique SGC cell line from a gastric cancer patient in whom an autopsy was performed. A new SGC cell line, OCUM-14, was established from malignant ascites of a male patient with SGC. A postmortem autopsy was performed on the patient. Characterization of OCUM-14 cells was analyzed by microscopic examination, reverse transcription polymerase chain reaction, fluorescence in situ hybridization analysis, immunohistochemical examination, CCK-8 assay, and in vivo assay. OCUM-14 cells grew singly or in clusters, and were floating and round-shaped. Most OCUM-14 cells had many microvilli on their surfaces. The doubling time was 43.1 h, and the subcutaneous inoculation of 1.0 × 10 A new gastric cancer cell line, OCUM-14, was established from SGC and showed FGFR2 overexpression. OCUM-14 might be useful for elucidating the characteristic mechanisms of SGC and clarifying the effect of FGFR2 inhibitors on SGC.
Sections du résumé
BACKGROUND
BACKGROUND
The prognosis of scirrhous gastric carcinoma (SGC), which is characterized by rapid infiltration and proliferation of cancer cells accompanied by extensive stromal fibrosis, is extremely poor. In this study, we report the establishment of a unique SGC cell line from a gastric cancer patient in whom an autopsy was performed.
METHODS
METHODS
A new SGC cell line, OCUM-14, was established from malignant ascites of a male patient with SGC. A postmortem autopsy was performed on the patient. Characterization of OCUM-14 cells was analyzed by microscopic examination, reverse transcription polymerase chain reaction, fluorescence in situ hybridization analysis, immunohistochemical examination, CCK-8 assay, and in vivo assay.
RESULTS
RESULTS
OCUM-14 cells grew singly or in clusters, and were floating and round-shaped. Most OCUM-14 cells had many microvilli on their surfaces. The doubling time was 43.1 h, and the subcutaneous inoculation of 1.0 × 10
CONCLUSION
CONCLUSIONS
A new gastric cancer cell line, OCUM-14, was established from SGC and showed FGFR2 overexpression. OCUM-14 might be useful for elucidating the characteristic mechanisms of SGC and clarifying the effect of FGFR2 inhibitors on SGC.
Identifiants
pubmed: 30652228
doi: 10.1245/s10434-018-07145-2
pii: 10.1245/s10434-018-07145-2
doi:
Substances chimiques
Antineoplastic Agents
0
FGFR2 protein, human
EC 2.7.10.1
Receptor, Fibroblast Growth Factor, Type 2
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1093-1102Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP18H02883
Organisme : Japan Society for the Promotion of Science
ID : JP23390329