C-type natriuretic peptide regulates sperm capacitation by the cGMP/PKG signalling pathway via Ca
Animals
Calcium
/ metabolism
Cervix Uteri
/ metabolism
Cyclic GMP
/ metabolism
Cyclic GMP-Dependent Protein Kinases
/ metabolism
Female
Humans
Male
Natriuretic Peptide, C-Type
/ metabolism
Oviducts
/ metabolism
Phosphorylation
Rats
Rats, Sprague-Dawley
Receptors, Atrial Natriuretic Factor
/ metabolism
Signal Transduction
/ physiology
Sperm Capacitation
/ physiology
Spermatozoa
/ metabolism
Tyrosine
/ metabolism
C-type natriuretic peptide
CGMP/PKG signalling pathway
Ca(2+)
Sperm capacitation
Tyrosine phosphorylation
Journal
Reproductive biomedicine online
ISSN: 1472-6491
Titre abrégé: Reprod Biomed Online
Pays: Netherlands
ID NLM: 101122473
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
20
02
2018
revised:
21
11
2018
accepted:
23
11
2018
pubmed:
19
1
2019
medline:
31
3
2020
entrez:
19
1
2019
Statut:
ppublish
Résumé
What is the effect of C-type natriuretic peptide (CNP) on human sperm capacitation in vitro and what is the mechanism of this effect? CNP/NPR-B expression in the female rat genital tract was examined by immunohistochemistry and western blot assay, and then the role of CNP in human sperm capacitation was determined. The signal transduction pathway of CNP in the process was determined to elucidate the regulation mechanism of CNP by enzyme-linked immunosorbent assay and flow cytometry. Both CNP and NPR-B were expressed in the genital tract of female rats, especially in the mucosa epithelium cell of the oviduct; the CNP level in the rat oviduct was higher than that in the cervix. Both CNP and NPR-B level in the rat oviduct varied during the oestrus cycle, maximal expression being observed at proestrus. Furthermore, intracellular cGMP level in spermatozoa was significantly enhanced by CNP (P < 0.01). PKG activity was detected in the spermatozoa, and it can be activated by the CNP and 8-Br-cGMP (cGMP analogue). The PKG inhibitor KT5823 inhibited the effect of CNP on sperm hyperactivation and the acrosome reaction. Finally, Ca CNP secreted by the female genital tract might bind to NPR-B on the spermatozoa. It successively stimulated intracellular cGMP/PKG signalling, increased Ca
Identifiants
pubmed: 30655075
pii: S1472-6483(19)30003-3
doi: 10.1016/j.rbmo.2018.11.025
pii:
doi:
Substances chimiques
Natriuretic Peptide, C-Type
127869-51-6
Tyrosine
42HK56048U
Cyclic GMP-Dependent Protein Kinases
EC 2.7.11.12
Receptors, Atrial Natriuretic Factor
EC 4.6.1.2
atrial natriuretic factor receptor C
EC 4.6.1.2
Cyclic GMP
H2D2X058MU
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
289-299Informations de copyright
Copyright © 2019 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.