RANKL is associated with persistent primary teeth and delayed permanent tooth emergence.
children
delayed tooth eruption
genetic polymorphism
persistent primary teeth
tooth eruption
Journal
International journal of paediatric dentistry
ISSN: 1365-263X
Titre abrégé: Int J Paediatr Dent
Pays: England
ID NLM: 9107511
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
02
07
2018
revised:
13
11
2018
accepted:
09
01
2019
pubmed:
19
1
2019
medline:
30
10
2019
entrez:
19
1
2019
Statut:
ppublish
Résumé
Tooth eruption is a process that is not fully understood. To evaluate whether genetic polymorphisms for RANK/RANKL/OPG are associated with delayed tooth emergence. To evaluate whether the relative expression of this genes is associated with persistent primary teeth. To evaluate whether genetic polymorphisms for RANK/RANKL/OPG could be involved in delayed tooth emergence, saliva samples from 160 children, aged 6-13 years old, were analysed. To test if there is correlation between gene expression of RANK/RANKL/OPG in children with delayed tooth emergence and persistent primary teeth, periapical tissue from 15 children with persistent primary teeth and from 15 control subjects were collected for qPCR analysis. Fifty-six children with delayed tooth emergence (35%) had at least one permanent tooth with delayed emergence. The T allele in RANKL (rs9594738) increased the risk of delayed tooth emergence (P = 0.02; OR = 1.71, 95%CI 1.09-2.75). The relative gene expression for RANKL and the ratio RANKL/OPG in children with delayed tooth emergence and persistent primary teeth were lower compared to controls (P = 0.02 and P = 0.005, respectively). Data suggest that the polymorphism rs9594738 in RANKL is associated with delayed permanent tooth emergence. Moreover, reduced relative gene expression of RANKL in periapical tissue is associated with persistent primary teeth.
Sections du résumé
BACKGROUND
BACKGROUND
Tooth eruption is a process that is not fully understood.
AIM
OBJECTIVE
To evaluate whether genetic polymorphisms for RANK/RANKL/OPG are associated with delayed tooth emergence. To evaluate whether the relative expression of this genes is associated with persistent primary teeth.
DESIGN
METHODS
To evaluate whether genetic polymorphisms for RANK/RANKL/OPG could be involved in delayed tooth emergence, saliva samples from 160 children, aged 6-13 years old, were analysed. To test if there is correlation between gene expression of RANK/RANKL/OPG in children with delayed tooth emergence and persistent primary teeth, periapical tissue from 15 children with persistent primary teeth and from 15 control subjects were collected for qPCR analysis.
RESULTS
RESULTS
Fifty-six children with delayed tooth emergence (35%) had at least one permanent tooth with delayed emergence. The T allele in RANKL (rs9594738) increased the risk of delayed tooth emergence (P = 0.02; OR = 1.71, 95%CI 1.09-2.75). The relative gene expression for RANKL and the ratio RANKL/OPG in children with delayed tooth emergence and persistent primary teeth were lower compared to controls (P = 0.02 and P = 0.005, respectively).
CONCLUSIONS
CONCLUSIONS
Data suggest that the polymorphism rs9594738 in RANKL is associated with delayed permanent tooth emergence. Moreover, reduced relative gene expression of RANKL in periapical tissue is associated with persistent primary teeth.
Substances chimiques
Osteoprotegerin
0
Types de publication
Journal Article
Langues
eng
Pagination
294-300Subventions
Organisme : Coordination of Improvement of Higher Level Personnel (CAPES)
Organisme : São Paulo Research Foundation (FAPESP)
ID : 2015/06866-5
Organisme : São Paulo Research Foundation (FAPESP)
ID : 2015/09034-0
Informations de copyright
© 2019 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.