14-3-3ζ promotes gliomas cells invasion by regulating Snail through the PI3K/AKT signaling.
14-3-3 Proteins
/ genetics
Animals
Brain
/ metabolism
Brain Neoplasms
/ genetics
Cell Movement
Cell Proliferation
Female
Glioma
/ genetics
Humans
Male
Mice, Nude
Middle Aged
Phosphatidylinositol 3-Kinases
/ metabolism
Proto-Oncogene Proteins c-akt
/ metabolism
RNA, Messenger
/ metabolism
Signal Transduction
Snail Family Transcription Factors
/ genetics
14-3-3ζ
PI3K/AKT
Snail
gliomas
invasion
Journal
Cancer medicine
ISSN: 2045-7634
Titre abrégé: Cancer Med
Pays: United States
ID NLM: 101595310
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
08
08
2018
revised:
12
11
2018
accepted:
10
12
2018
pubmed:
19
1
2019
medline:
27
3
2020
entrez:
19
1
2019
Statut:
ppublish
Résumé
14-3-3ζ has been reported to function as critical regulators of diverse cellular responses. However, the role of 14-3-3ζ in gliomas progression remains largely unknown. The expression level of 14-3-3ζ and Snail was detected by Western blot analysis and quantitative polymerase chain reaction in different grades of human gliomas. The effect of 14-3-3ζ on gliomas progression was measured using cell migration and invasion assay, the colony formation experiment, and CCK-8 assay. The effect of 14-3-3ζ on PI3K/AKT/Snail signaling protein expression levels was tested by Western blotting. Firstly, 14-3-3ζ was often up-regulated in high-grade gliomas relative to low-grade gliomas, and this overexpression was significantly related to tumor size, Karnofsky Performance Scale score and weaker disease-free survival. Secondly, the overexpression of 14-3-3ζ promoted gliomas cells proliferation, migration, and invasion. Conversely, the knockdown of 14-3-3ζ suppressed gliomas cells proliferation, migration, and invasion. Furthermore, subsequent mechanistic studies showed that 14-3-3ζ could activate PI3K/AKT/Snail signaling pathway to facilitate gliomas cells proliferation, migration, and invasion. This study shows that the overexpression of 14-3-3ζ can promote remarkably gliomas cells proliferation, migration, and invasion by regulating the Snail protein expression through activating PI3K/AKT signaling, and it may serve as a potential prognostic marker and therapeutic target for gliomas.
Identifiants
pubmed: 30656845
doi: 10.1002/cam4.1950
pmc: PMC6382716
doi:
Substances chimiques
14-3-3 Proteins
0
RNA, Messenger
0
Snail Family Transcription Factors
0
YWHAZ protein, human
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Retracted Publication
Langues
eng
Sous-ensembles de citation
IM
Pagination
783-794Commentaires et corrections
Type : RetractionIn
Informations de copyright
© 2019 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
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