Spatial and temporal changes in myogenic protein expression by the microenvironment after freeze injury.
Animals
Cell Differentiation
/ physiology
Freezing
Immunohistochemistry
/ methods
Muscle Development
/ physiology
Muscle Proteins
/ metabolism
Muscle, Skeletal
/ pathology
Myogenic Regulatory Factors
/ metabolism
Myogenin
/ biosynthesis
PAX3 Transcription Factor
/ metabolism
PAX7 Transcription Factor
/ metabolism
Rats
Regeneration
/ physiology
Wounds and Injuries
p38 Mitogen-Activated Protein Kinases
/ metabolism
Pax
microenvironment
muscle regeneration
myogenic regulatory factor
myogenic stem cells
Journal
Journal of anatomy
ISSN: 1469-7580
Titre abrégé: J Anat
Pays: England
ID NLM: 0137162
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
accepted:
15
11
2018
pubmed:
19
1
2019
medline:
6
6
2020
entrez:
19
1
2019
Statut:
ppublish
Résumé
Skeletal muscle has the remarkable capability to regenerate itself following injury. Adult myogenic stem cells (MSCs) are responsible for the repair and regeneration, and their activity is controlled by intrinsic and extrinsic factors. The aim of this study was to examine and compare the expression levels of Pax3, Pax7, MRF and p38 proteins during the course of regeneration and in different areas of the focal freeze-lesion damaged adult rat TA muscle. Using the focal freeze injury model, immunohistochemistry, laser-capture micro-dissection and Western blot analysis were performed. The results show that (1) in the severely damaged area, the focal freeze-lesion injury significantly activated Pax7 and myogenin expression within 7 days and down-regulated Pax3, MyoD and Myf-5 within 1 or 3 days, and (2) the level of the p38 protein was strongly and transiently up-regulated in the whole muscle on day 7 following injury, whereas the level of the pp38 protein was down-regulated within 3 days in the severely damaged and non-damaged areas. These findings indicate that the temporal (e.g. the time course of regeneration) and spatial (e.g. three zones created by the focal freeze-lesion) cues in a regenerating muscle have a significant impact on the activity of the adult MSCs.
Identifiants
pubmed: 30657171
doi: 10.1111/joa.12925
pmc: PMC6365476
doi:
Substances chimiques
Muscle Proteins
0
Myogenic Regulatory Factors
0
Myogenin
0
PAX3 Transcription Factor
0
PAX7 Transcription Factor
0
p38 Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
359-367Informations de copyright
© 2019 Anatomical Society.
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