Characterization of Plasma-Derived Extracellular Vesicles Isolated by Different Methods: A Comparison Study.
enrichment
extracellular vesicles
lateral flow immunoassay
nanoparticle tracking analysis
ultracentrifugation
Journal
Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056
Informations de publication
Date de publication:
17 Jan 2019
17 Jan 2019
Historique:
received:
30
11
2018
revised:
08
01
2019
accepted:
11
01
2019
entrez:
20
1
2019
pubmed:
20
1
2019
medline:
20
1
2019
Statut:
epublish
Résumé
Extracellular vesicles (EV) are small membrane structures released by cells that act as potent mediators of intercellular communication. The study of EV biology is important, not only to strengthen our knowledge of their physiological roles, but also to better understand their involvement in several diseases. In the field of biomedicine they have been studied as a novel source of biomarkers and drug delivery vehicles. The most commonly used method for EV enrichment in crude pellet involves serial centrifugation and ultracentrifugation. Recently, different protocols and techniques have been developed to isolate EV that imply less time and greater purification. Here we carry out a comparative analysis of three methods to enrich EV from plasma of healthy controls: ultracentrifugation, ExoQuick
Identifiants
pubmed: 30658418
pii: bioengineering6010008
doi: 10.3390/bioengineering6010008
pmc: PMC6466225
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministerio de Economía y Competitividad
ID : CTQ2013-47396-R
Organisme : Ministerio de Economía y Competitividad
ID : MAT2017-84959-C2-1-R
Organisme : Consejería de Economía y Empleo del Principado de Asturias
ID : GRUPIN14-022
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