An evolutionarily-conserved Wnt3/β-catenin/Sp5 feedback loop restricts head organizer activity in Hydra.
Animals
Biological Evolution
Body Patterning
/ genetics
Gene Expression Regulation, Developmental
Head
/ growth & development
Hydra
/ genetics
Intercellular Signaling Peptides and Proteins
/ genetics
Planarians
/ genetics
RNA Interference
Regeneration
/ physiology
Signal Transduction
Wnt3 Protein
/ genetics
Zebrafish
/ embryology
beta Catenin
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 01 2019
18 01 2019
Historique:
received:
20
03
2018
accepted:
17
12
2018
entrez:
20
1
2019
pubmed:
20
1
2019
medline:
14
2
2019
Statut:
epublish
Résumé
Polyps of the cnidarian Hydra maintain their adult anatomy through two developmental organizers, the head organizer located apically and the foot organizer basally. The head organizer is made of two antagonistic cross-reacting components, an activator, driving apical differentiation and an inhibitor, preventing ectopic head formation. Here we characterize the head inhibitor by comparing planarian genes down-regulated when β-catenin is silenced to Hydra genes displaying a graded apical-to-basal expression and an up-regulation during head regeneration. We identify Sp5 as a transcription factor that fulfills the head inhibitor properties: leading to a robust multiheaded phenotype when knocked-down in Hydra, acting as a transcriptional repressor of Wnt3 and positively regulated by Wnt/β-catenin signaling. Hydra and zebrafish Sp5 repress Wnt3 promoter activity while Hydra Sp5 also activates its own expression, likely via β-catenin/TCF interaction. This work identifies Sp5 as a potent feedback loop inhibitor of Wnt/β-catenin signaling, a function conserved across eumetazoan evolution.
Identifiants
pubmed: 30659200
doi: 10.1038/s41467-018-08242-2
pii: 10.1038/s41467-018-08242-2
pmc: PMC6338789
doi:
Substances chimiques
Intercellular Signaling Peptides and Proteins
0
Wnt3 Protein
0
beta Catenin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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