Detection of Concordance between Transcriptional Levels of GPCRs and Receptor-Activity-Modifying Proteins.
Biological Sciences
Cell Biology
Molecular Biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
25 Jan 2019
25 Jan 2019
Historique:
received:
31
01
2018
revised:
04
09
2018
accepted:
21
12
2018
pubmed:
20
1
2019
medline:
20
1
2019
entrez:
20
1
2019
Statut:
ppublish
Résumé
A recent phylogenetic analysis showed global co-evolution of G protein-coupled receptors (GPCRs) and receptor-activity-modifying proteins (RAMPs) suggesting global interactions between these two protein families. Experimental validation of these findings is challenging because in humans whereas there are only three genes encoding RAMPs, there are about 800 genes encoding GPCRs. Here, we report an experimental approach to evaluate GPCR-RAMP interactions. As a proof-of-concept experiment, we over-expressed RAMP2 in HEK293T cells and evaluated the effect on the transcriptional levels of 14 representative GPCRs that were selected based on the earlier phylogenetic analysis. We utilized a multiplexed error-correcting fluorescence in situ hybridization (MERFISH) method to detect message levels for individual GPCRs in single cells. The MERFISH results showed changes in GPCR message levels with RAMP2 over-expression in a concordant pattern that was predicted by the earlier phylogenetic analysis. These results provide additional evidence that GPCR-RAMP interactions are more widespread than previously appreciated and that these interactions have functional consequences.
Identifiants
pubmed: 30660104
pii: S2589-0042(18)30254-2
doi: 10.1016/j.isci.2018.12.024
pmc: PMC6354700
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
366-374Informations de copyright
Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
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