Creation of a Porcine Kyphotic Model.
Animal model
Early-onset scoliosis
Immature spine
Pig
Thoracic hyperkyphosis
Journal
Spine deformity
ISSN: 2212-1358
Titre abrégé: Spine Deform
Pays: England
ID NLM: 101603979
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
04
10
2017
revised:
02
07
2018
accepted:
07
07
2018
entrez:
21
1
2019
pubmed:
21
1
2019
medline:
3
7
2019
Statut:
ppublish
Résumé
Large animal study. Create a thoracic hyperkyphotic deformity in an immature porcine spine, so that future researchers may use this model to validate spinal instrumentation and other therapies used in the treatment of hyperkyphosis. Although several scoliotic animal models have been developed, there have been no reports of a thoracic hyperkyphotic animal model creation in an immature animal. The present study was designed to produce a porcine hyperkyphotic model by the time the pig weighed 25 kg, which corresponds to the approximate weight of a child undergoing surgery for early-onset scoliosis (EOS). Successful surgical procedures were performed in 6 consecutive 10-kg (male, 5-week-old) immature Yorkshire pigs. Procedure protocol consisted of 1) a left thoracotomy at T10-T11, 2) screw placement at T9 and T11, 3) partial vertebrectomy at T10, 4) posterior interspinous ligament transection, and 5) placement of wire loop around screws and tightening. Weekly x-ray imaging was performed preoperatively and postoperatively, documenting progressively increasing kyphosis as the pig grew. Necropsy was performed 5-6 weeks after surgery, with CT, slab section, and histologic analysis. Average T9-T11 kyphosis (measured by sagittal Cobb angle) was 6.1° ± 1.4° (mean ± SD) preoperatively, 30.5° ± 1.0° immediately postoperation, and significantly increased to 50.3° ± 7.2° (p < .0001) over 5-6 weeks in 6 consecutive pigs at time of necropsy. An animal model of relatively more rigid-appearing thoracic hyperkyphotic deformities in immature pigs has been created. Subsequent studies addressing management of early-onset kyphosis with spinal instrumentation are now possible. Level V.
Sections du résumé
STUDY DESIGN
Large animal study.
OBJECTIVE
Create a thoracic hyperkyphotic deformity in an immature porcine spine, so that future researchers may use this model to validate spinal instrumentation and other therapies used in the treatment of hyperkyphosis.
SUMMARY OF BACKGROUND DATA
Although several scoliotic animal models have been developed, there have been no reports of a thoracic hyperkyphotic animal model creation in an immature animal. The present study was designed to produce a porcine hyperkyphotic model by the time the pig weighed 25 kg, which corresponds to the approximate weight of a child undergoing surgery for early-onset scoliosis (EOS).
METHODS
Successful surgical procedures were performed in 6 consecutive 10-kg (male, 5-week-old) immature Yorkshire pigs. Procedure protocol consisted of 1) a left thoracotomy at T10-T11, 2) screw placement at T9 and T11, 3) partial vertebrectomy at T10, 4) posterior interspinous ligament transection, and 5) placement of wire loop around screws and tightening. Weekly x-ray imaging was performed preoperatively and postoperatively, documenting progressively increasing kyphosis as the pig grew. Necropsy was performed 5-6 weeks after surgery, with CT, slab section, and histologic analysis.
RESULTS
Average T9-T11 kyphosis (measured by sagittal Cobb angle) was 6.1° ± 1.4° (mean ± SD) preoperatively, 30.5° ± 1.0° immediately postoperation, and significantly increased to 50.3° ± 7.2° (p < .0001) over 5-6 weeks in 6 consecutive pigs at time of necropsy.
CONCLUSIONS
An animal model of relatively more rigid-appearing thoracic hyperkyphotic deformities in immature pigs has been created. Subsequent studies addressing management of early-onset kyphosis with spinal instrumentation are now possible.
LEVEL OF EVIDENCE
Level V.
Identifiants
pubmed: 30660214
pii: S2212-134X(18)30138-2
doi: 10.1016/j.jspd.2018.07.002
pmc: PMC7341554
mid: NIHMS1601499
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
213-219Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM121342
Pays : United States
Organisme : NIDCR NIH HHS
ID : T32 DE017551
Pays : United States
Informations de copyright
Copyright © 2018 Scoliosis Research Society. Published by Elsevier Inc. All rights reserved.
Références
Adv Nutr. 2014 Sep;5(5):503-14
pubmed: 25469382
J Trace Elem Med Biol. 2015;31:148-56
pubmed: 25456335
Spine (Phila Pa 1976). 2005 Sep 1;30(17 Suppl):S69-74
pubmed: 16138068
Spine J. 2008 Nov-Dec;8(6):933-9
pubmed: 18082463
Spine (Phila Pa 1976). 1999 Oct 15;24(20):2139-46
pubmed: 10543013
Spine (Phila Pa 1976). 2015 Mar 1;40(5):305-11
pubmed: 25901978
Acta Orthop Scand Suppl. 1962;59:1-26
pubmed: 13928602
Eur Spine J. 2013 Jul;22(7):1576-82
pubmed: 23645228
Spine (Phila Pa 1976). 2005 Nov 1;30(21):2388-92
pubmed: 16261114
Spine (Phila Pa 1976). 2013 Apr 15;38(8):E464-8
pubmed: 23370680
Spine (Phila Pa 1976). 2012 Jul 1;37(15):1303-9
pubmed: 22210014
J Orthop Res. 2015 Apr;33(4):458-67
pubmed: 25492698
J Spinal Disord Tech. 2014 Dec;27(8):E294-300
pubmed: 25374380
APMIS. 2005 Oct;113(10):702-7
pubmed: 16309430
J Pediatr Orthop. 2016 Jun;36(4):336-42
pubmed: 25851684
Spine (Phila Pa 1976). 2009 Jul 1;34(15):E501-6
pubmed: 19564754
Eur Spine J. 2002 Apr;11(2):137-44
pubmed: 11956920
Spine (Phila Pa 1976). 2002 Apr 1;27(7):689-93
pubmed: 11923660