Upregulation of miR‑33b promotes endometriosis via inhibition of Wnt/β‑catenin signaling and ZEB1 expression.
Animals
Cell Line, Tumor
Cell Proliferation
/ genetics
Disease Models, Animal
Endometriosis
/ drug therapy
Female
Gene Expression Regulation
/ drug effects
Humans
MicroRNAs
/ genetics
RNA, Small Interfering
/ genetics
Rats
Transcriptional Activation
/ drug effects
Wnt Proteins
/ agonists
Wnt Signaling Pathway
/ drug effects
Zinc Finger E-box-Binding Homeobox 1
/ genetics
beta Catenin
/ genetics
Journal
Molecular medicine reports
ISSN: 1791-3004
Titre abrégé: Mol Med Rep
Pays: Greece
ID NLM: 101475259
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
19
11
2017
accepted:
30
07
2018
pubmed:
22
1
2019
medline:
24
5
2019
entrez:
22
1
2019
Statut:
ppublish
Résumé
The present study aimed to investigate the role and mechanisms of microRNA (miR)‑33b in endometriosis (Ems). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), MTT assays, flow cytometry, caspase‑3/9 activity assays and western blotting were performed in the present study. Initially, miR‑33b expression in an Ems rat model was investigated by RT‑qPCR and was demonstrated to be upregulated in Ems tissue samples of rats compared with the control group. In addition, miR‑33b upregulation inhibited cell growth and enhanced apoptosis in an Ems model (primary cell cultures) compared with the control group. In addition, miR‑33b up‑regulation reduced Wnt/β‑catenin signaling pathway and suppressed zinc finger E‑box‑binding homeobox 1 (ZEB1) protein expression in the in vitro Ems model (primary cell cultures) compared with the control group. Furthermore, small interfering‑ZEB1 ameliorated the effects of miR‑33b downregulation on Ems cell growth in the in vitro Ems model. Additionally, a Wnt agonist reduced the effects of miR‑33b upregulation on Ems cell growth in the in vitro Ems model. In conclusion, the present study demonstrated that upregulation of miR‑33b may promote Ems through Wnt/β‑catenin by ZEB1 expression.
Identifiants
pubmed: 30664209
doi: 10.3892/mmr.2019.9870
pmc: PMC6390049
doi:
Substances chimiques
CTNNB1 protein, human
0
MicroRNAs
0
RNA, Small Interfering
0
Wnt Proteins
0
ZEB1 protein, human
0
Zinc Finger E-box-Binding Homeobox 1
0
beta Catenin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2144-2152Références
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