Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres.
Crestospheres
Multipotency, self-renewal
Neural crest stem cells
Stem cell maintenance
Trunk neural crest
Journal
Developmental biology
ISSN: 1095-564X
Titre abrégé: Dev Biol
Pays: United States
ID NLM: 0372762
Informations de publication
Date de publication:
15 03 2019
15 03 2019
Historique:
received:
17
08
2018
revised:
09
01
2019
accepted:
09
01
2019
pubmed:
22
1
2019
medline:
5
9
2019
entrez:
22
1
2019
Statut:
ppublish
Résumé
Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.
Identifiants
pubmed: 30664880
pii: S0012-1606(18)30558-X
doi: 10.1016/j.ydbio.2019.01.010
pmc: PMC6497816
mid: NIHMS1519377
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
137-146Subventions
Organisme : NICHD NIH HHS
ID : F32 HD087026
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE024157
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE027568
Pays : United States
Informations de copyright
Published by Elsevier Inc.
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