Stiffness measurement of nanosized liposomes using solid-state nanopore sensor with automated recapturing platform.
Liposome
Nanoparticle
Recapturing platform
Solid-state nanopore
Stiffness
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
13
11
2018
revised:
21
12
2018
accepted:
07
01
2019
pubmed:
23
1
2019
medline:
11
1
2020
entrez:
23
1
2019
Statut:
ppublish
Résumé
This paper describes a method to gauge the stiffness of nanosized liposomes - a nanoscale vesicle - using a custom-made recapture platform coupled to a solid-state nanopore sensor. The recapture platform electrically profiles a given liposome vesicle multiple times through automated reversal of the voltage polarity immediately following a translocation instance to re-translocate the same analyte through the nanopore - provides better statistical insight at the molecular level by analyzing the same particle multiple times compared to conventional nanopore platforms. The capture frequency depends on the applied voltage with lower voltages (i.e., 100 mV) permitting higher recapture instances than at higher voltages (>200 mV) since the probability of particles exiting the nanopore capture radius increases with voltage. The shape deformation was inferred by comparing the normalized relative current blockade (
Identifiants
pubmed: 30667089
doi: 10.1002/elps.201800476
doi:
Substances chimiques
Liposomes
0
Polystyrenes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1337-1344Subventions
Organisme : National Institute of Health
ID : R03EB022759
Pays : International
Organisme : National Science Foundation
ID : CMMI #1712069
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.