High-Throughput Bioanalysis of Bevacizumab in Human Plasma Based on Enzyme-Linked Aptamer Assay Using Anti-Idiotype DNA Aptamer.

Aptamers, Nucleotide / chemistry Bevacizumab / blood Enzyme-Linked Immunosorbent Assay High-Throughput Screening Assays Horseradish Peroxidase / chemistry Humans Staphylococcal Protein A / chemistry

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
19 02 2019
Historique:
pubmed: 23 1 2019
medline: 15 8 2020
entrez: 23 1 2019
Statut: ppublish

Résumé

We propose a highly selective, sensitive, accurate, and high-throughput bioanalysis method for bevacizumab utilizing an anti-idiotype DNA aptamer. With this method, bevacizumab in a plasma sample was reacted in a 96-well plate immobilized with the aptamer and further reacted with a protein A-HRP conjugate. The resulting HRP activity was colorimetrically detected using a microplate reader. The calibration curve of bevacizumab ranged from 0.05 to 5.0 μg/mL, and showed a good correlation coefficient ( r

Identifiants

DOI: 10.1021/acs.analchem.8b05725 PMID: 30667211
pubmed: 30667211
doi: 10.1021/acs.analchem.8b05725
doi:

Substances chimiques

Aptamers, Nucleotide 0
Staphylococcal Protein A 0
Bevacizumab 2S9ZZM9Q9V
Horseradish Peroxidase EC 1.11.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3125-3130

Auteurs

Tomohiro Yamada (T)

Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences , University of Shizuoka , 52-1 Yada , Suruga-ku, Shizuoka 422-8526 , Japan.

Taro Saito (T)

Department of Biotechnology and Life Science, Graduate School of Engineering , Tokyo University of Agriculture and Technology , 2-24-16 Naka-cho , Koganei , Tokyo 184-8588 , Japan.

Yoshia Hill (Y)

Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences , University of Shizuoka , 52-1 Yada , Suruga-ku, Shizuoka 422-8526 , Japan.

Yutaka Shimizu (Y)

Department of Biotechnology and Life Science, Graduate School of Engineering , Tokyo University of Agriculture and Technology , 2-24-16 Naka-cho , Koganei , Tokyo 184-8588 , Japan.

Kaori Tsukakoshi (K)

Department of Biotechnology and Life Science, Graduate School of Engineering , Tokyo University of Agriculture and Technology , 2-24-16 Naka-cho , Koganei , Tokyo 184-8588 , Japan.

Hajime Mizuno (H)

Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences , University of Shizuoka , 52-1 Yada , Suruga-ku, Shizuoka 422-8526 , Japan.

Hideki Hayashi (H)

Laboratory of Pharmacy Practice and Social Science , Gifu Pharmaceutical University , Daigaku-nishi 1-25-4 , Gifu 501-1196 , Japan.

Kazunori Ikebukuro (K)

Department of Biotechnology and Life Science, Graduate School of Engineering , Tokyo University of Agriculture and Technology , 2-24-16 Naka-cho , Koganei , Tokyo 184-8588 , Japan.
ORCID: 0000-0003-2838-0562

Toshimasa Toyo'oka (T)

Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences , University of Shizuoka , 52-1 Yada , Suruga-ku, Shizuoka 422-8526 , Japan.

Kenichiro Todoroki (K)

Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences , University of Shizuoka , 52-1 Yada , Suruga-ku, Shizuoka 422-8526 , Japan.
ORCID: 0000-0001-8629-8829

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › High-Throughput Bioanalysis of Bevacizumab in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Anticorps monoclonaux humanisés › Bévacizumab › High-Throughput Bioanalysis of Bevacizumab in...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › High-Throughput Bioanalysis of Bevacizumab in...
questionsmedicales.fr › Techniques d'investigation › Tests de criblage à haut débit › High-Throughput Bioanalysis of Bevacizumab in...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Peroxidases › Horseradish peroxidase › High-Throughput Bioanalysis of Bevacizumab in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › High-Throughput Bioanalysis of Bevacizumab in...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes bactériens › Protéine A staphylococcique › High-Throughput Bioanalysis of Bevacizumab in...