Validation of an analytical method to quantify the permeation and penetration of flurbiprofen into human pharynx tissue.
Franz cell
HPLC
flurbiprofen
pharyngitis
pharynx
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
21
08
2018
revised:
14
11
2018
accepted:
17
01
2019
pubmed:
23
1
2019
medline:
6
8
2019
entrez:
23
1
2019
Statut:
ppublish
Résumé
The aim of this investigation was to develop receiver and extraction fluids, and subsequently validate an analytical method to quantify the permeation and penetration of flurbiprofen into human pharynx tissue using a Franz diffusion cell. The solubility and stability of flurbiprofen in a suitable receiver fluid, and a suitable extraction method and fluid to recover and quantitate flurbiprofen from human pharynx tissue, were investigated using high-performance liquid chromatography (HPLC). The potential interference of human pharynx tissue in the receiver fluid was also investigated. The HPLC analytical method was successfully validated according to current guidelines. The final receiver fluid demonstrated sufficient solubility and stability, and the extraction method and fluid resulted in >95% recovery of flurbiprofen following exposure to human pharynx tissue. The lower limit of quantitation of flurbiprofen was 0.045 μg/mL in both the receiver and extraction fluids. There was no interference of the human pharynx tissue with the HPLC method. This investigation validated an analytical method for quantitating flurbiprofen, and determined a suitable receiver fluid and extraction method and fluid, which can be used to investigate the permeation and penetration of flurbiprofen through human pharynx tissue using the Franz diffusion cell method.
Identifiants
pubmed: 30667527
doi: 10.1002/bmc.4499
pmc: PMC6618079
doi:
Substances chimiques
Saline Solution
0
Water
059QF0KO0R
Ethanol
3K9958V90M
Flurbiprofen
5GRO578KLP
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Pagination
e4499Informations de copyright
© 2019 John Wiley & Sons, Ltd.
Références
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Nov 15;971:58-63
pubmed: 25264913
Eur J Gen Pract. 2016 Jun;22(2):111-8
pubmed: 27028316
Pain Manag. 2018 Mar;8(2):85-94
pubmed: 29381110
J Adv Pharm Technol Res. 2011 Jul;2(3):151-5
pubmed: 22171311
J Chromatogr Sci. 2015 Oct;53(9):1443-8
pubmed: 25820260
J Pain Res. 2017 Jul 06;10:1591-1600
pubmed: 28740426
Exp Anim. 2011;60(4):373-84
pubmed: 21791877
BMC Infect Dis. 2017 May 17;17(1):350
pubmed: 28514947
J Helminthol. 2003 Mar;77(1):27-31
pubmed: 12590661
J Pharm Sci. 1991 Sep;80(9):820-3
pubmed: 1800701
Br J Pain. 2018 Nov;12(4):208-216
pubmed: 30349694
AAPS PharmSciTech. 2009;10(1):258-65
pubmed: 19280347
Biomed Chromatogr. 2017 Dec;31(12):
pubmed: 28623841
J Invest Dermatol. 1975 Mar;64(3):190-5
pubmed: 123263
Clin Pharmacol Ther. 1988 Aug;44(2):225-31
pubmed: 3293876
AAPS PharmSciTech. 2010 Sep;11(3):1432-41
pubmed: 20842539
BMC Vet Res. 2017 Nov 21;13(1):345
pubmed: 29162115
Carcinogenesis. 2006 Jan;27(1):137-45
pubmed: 16000402
Drug Metab Pharmacokinet. 2002;17(2):142-9
pubmed: 15618663
Biomed Chromatogr. 2016 Aug;30(8):1263-9
pubmed: 26686197
Biomed Chromatogr. 2019 Aug;33(8):e4499
pubmed: 30667527
Pain. 2014 Feb;155(2):422-8
pubmed: 24231654
J Chromatogr A. 2014 Nov 7;1367:1-8
pubmed: 25278162