Evaluation of phyto-medicinal efficacy of thymoquinone against Arsenic induced mitochondrial dysfunction and cytotoxicity in SH-SY5Y cells.
Antioxidants
/ pharmacology
Apoptosis
/ drug effects
Arsenic Trioxide
/ toxicity
Benzoquinones
/ pharmacology
Cell Line, Tumor
Cell Survival
/ drug effects
Humans
Mitochondria
/ drug effects
Neuroblastoma
/ drug therapy
Neurons
/ drug effects
Neuroprotective Agents
/ pharmacology
Nigella sativa
/ chemistry
Poly (ADP-Ribose) Polymerase-1
/ metabolism
Reactive Oxygen Species
/ metabolism
Abbreviations: As, Arsenic
Apoptosis
As(2)O(3), Arsenic trioxide
DCFDA, 2′, 7′–dichlorofluorescein diacetate
Flow cytometry
MMP, Mitochondrial Membrane Potential
Mitochondria
ROS, Reactive Oxygen Species
Reactive Oxygen Species
Rh-123, Rhodamine-123
TQ, Thymoquinone
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
15 Feb 2019
15 Feb 2019
Historique:
received:
17
04
2018
accepted:
17
09
2018
pubmed:
23
1
2019
medline:
25
6
2019
entrez:
23
1
2019
Statut:
ppublish
Résumé
It is evaluated that a few million individuals worldwide are experiencing Arsenic (As) harmfulness coming about because of anthropogenic discharges. There is likewise proof to propose that As can affect the peripheral, as well as, the central nervous system (CNS). On the contrary, thymoquinone (TQ), a biologically active ingredient of Nigella sativa has exhibited numerous neuro-pharmacological traits since ancient times. In the present study, the neuroprotective efficacy of TQ was explored by primarily studying its antioxidant and anti-apoptotic potential against Arsenic trioxide (As For experimentation, cells were seeded in 96 well tissue culture plates and kept undisturbed for 24 h to attain proper adhesion. After 75-80% confluence, cells were pretreated with 10 µM and 20 µM thymoquinone (TQ) for 1 h After adding 2 µM As, cells were set aside for incubation for 24 h without changing the medium. The mitigatory effects of TQ with particular reference to cell viability and cytotoxicity, the generation of reactive oxygen species, DNA damage, and mitochondrial dynamics were studied. Pretreatment of SH-SY5Y cells with TQ (10 and 20 μM) for an hour and subsequent exposure to 2 μM As The findings of this study suggest that TQ may prevent neurotoxicity and As
Sections du résumé
BACKGROUND
BACKGROUND
It is evaluated that a few million individuals worldwide are experiencing Arsenic (As) harmfulness coming about because of anthropogenic discharges. There is likewise proof to propose that As can affect the peripheral, as well as, the central nervous system (CNS). On the contrary, thymoquinone (TQ), a biologically active ingredient of Nigella sativa has exhibited numerous neuro-pharmacological traits since ancient times.
HYPOTHESIS/PURPOSE
OBJECTIVE
In the present study, the neuroprotective efficacy of TQ was explored by primarily studying its antioxidant and anti-apoptotic potential against Arsenic trioxide (As
STUDY DESIGN
METHODS
For experimentation, cells were seeded in 96 well tissue culture plates and kept undisturbed for 24 h to attain proper adhesion. After 75-80% confluence, cells were pretreated with 10 µM and 20 µM thymoquinone (TQ) for 1 h After adding 2 µM As, cells were set aside for incubation for 24 h without changing the medium.
METHODS
METHODS
The mitigatory effects of TQ with particular reference to cell viability and cytotoxicity, the generation of reactive oxygen species, DNA damage, and mitochondrial dynamics were studied.
RESULTS
RESULTS
Pretreatment of SH-SY5Y cells with TQ (10 and 20 μM) for an hour and subsequent exposure to 2 μM As
CONCLUSION
CONCLUSIONS
The findings of this study suggest that TQ may prevent neurotoxicity and As
Identifiants
pubmed: 30668372
pii: S0944-7113(18)30481-1
doi: 10.1016/j.phymed.2018.09.197
pii:
doi:
Substances chimiques
Antioxidants
0
Benzoquinones
0
Neuroprotective Agents
0
Reactive Oxygen Species
0
PARP1 protein, human
EC 2.4.2.30
Poly (ADP-Ribose) Polymerase-1
EC 2.4.2.30
thymoquinone
O60IE26NUF
Arsenic Trioxide
S7V92P67HO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
224-230Informations de copyright
Copyright © 2018 Elsevier GmbH. All rights reserved.