Neutrophil-mediated antifungal activity against highly virulent Cryptococcus gattii strain R265.

Animals Cryptococcus gattii / immunology Cryptococcus neoformans / immunology Immunity, Cellular Mice, Inbred C57BL Microbial Viability Neutrophils / immunology Nitrosative Stress Oxidative Stress Phagocytosis
Cryptococcus gattii Cryptococcus neoformans neutrophils opsonization phagocytosis

Journal

Medical mycology
ISSN: 1460-2709
Titre abrégé: Med Mycol
Pays: England
ID NLM: 9815835

Informations de publication

Date de publication:
01 Nov 2019
Historique:
received: 25 09 2018
revised: 10 12 2018
accepted: 13 12 2018
pubmed: 23 1 2019
medline: 17 1 2020
entrez: 23 1 2019
Statut: ppublish

Résumé

Vaccine-induced immune responses, including neutrophil, macrophage, and T-cell responses, ameliorate cryptococcosis caused by Cryptococcus gattii. However, whether neutrophils can exert fungicidal activity against C. gattii remains to be elucidated. Therefore, in this study, we investigated the neutrophil-mediated fungicidal effect against C. gattii R265 in vitro and compared it to the related fungal pathogen, Cryptococcus neoformans standard strain H99. We found that neutrophils recognized, phagocytosed, and killed C. gattii R265 in the presence of fresh mouse serum. This antifungal effect required phagocytosis and serine protease activity but not nicotinamide adenine dinucleotide phosphate oxidase activity. We also demonstrated that C. gattii R265 was more resistant to oxidative and nitrosative stress than C. neoformans H99. Together, these findings indicate that neutrophils can exert fungicidal activity against highly virulent C. gattii, at least under in vitro conditions.

Identifiants

DOI: 10.1093/mmy/myy153 PMID: 30668754
pubmed: 30668754
pii: 5292626
doi: 10.1093/mmy/myy153
doi:

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1046-1054

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

Auteurs

Keigo Ueno (K)

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.

Nao Yanagihara (N)

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Yoshiko Otani (Y)

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Kiminori Shimizu (K)

Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

Yuki Kinjo (Y)

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Department of Bacteriology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Jikei Center for Biofilm Science and Technology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.

Yoshitsugu Miyazaki (Y)

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Cryptococcus › Cryptococcus gattii › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Cryptococcus › Cryptococcus neoformans › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité acquise › Immunité cellulaire › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Phénomènes microbiologiques › Viabilité microbienne › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Granulocytes neutrophiles › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress oxydatif › Stress nitrosatif › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress oxydatif › Neutrophil-mediated antifungal activity...
questionsmedicales.fr › Phénomènes du système immunitaire › Phagocytose › Neutrophil-mediated antifungal activity...