Hydrogen Indirectly Suppresses Increases in Hydrogen Peroxide in Cytoplasmic Hydroxyl Radical-Induced Cells and Suppresses Cellular Senescence.
Activating Transcription Factor 4
/ metabolism
Cellular Senescence
/ drug effects
Cyclin-Dependent Kinase Inhibitor p16
/ genetics
Cyclin-Dependent Kinase Inhibitor p21
/ genetics
Cytoplasm
/ metabolism
DNA Damage
Eukaryotic Initiation Factor-2
/ metabolism
Fibroblasts
/ drug effects
Gene Expression Regulation
/ drug effects
Glutathione
/ metabolism
Humans
Hydrogen
/ metabolism
Hydrogen Peroxide
/ metabolism
Hydroxyl Radical
/ metabolism
Lipid Peroxidation
Male
Oxidative Stress
Signal Transduction
/ drug effects
cellular senescence
cytoplasmic hydroxyl radical
hydrogen
hydrogen peroxide
lipid peroxide
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Jan 2019
21 Jan 2019
Historique:
received:
15
12
2018
revised:
16
01
2019
accepted:
18
01
2019
entrez:
24
1
2019
pubmed:
24
1
2019
medline:
2
5
2019
Statut:
epublish
Résumé
Bacteria inhabiting the human gut metabolize microbiota-accessible carbohydrates (MAC) contained in plant fibers and subsequently release metabolic products. Gut bacteria produce hydrogen (H₂), which scavenges the hydroxyl radical (•OH). Because H₂ diffuses within the cell, it is hypothesized that H₂ scavenges cytoplasmic •OH (cyto •OH) and suppresses cellular senescence. However, the mechanisms of cyto •OH-induced cellular senescence and the physiological role of gut bacteria-secreted H₂ have not been elucidated. Based on the pyocyanin-stimulated cyto •OH-induced cellular senescence model, the mechanism by which cyto •OH causes cellular senescence was investigated by adding a supersaturated concentration of H₂ into the cell culture medium. Cyto •OH-generated lipid peroxide caused glutathione (GSH) and heme shortage, increased hydrogen peroxide (H₂O₂), and induced cellular senescence via the phosphorylation of ataxia telangiectasia mutated kinase serine 1981 (p-ATM
Identifiants
pubmed: 30669692
pii: ijms20020456
doi: 10.3390/ijms20020456
pmc: PMC6359316
pii:
doi:
Substances chimiques
Cyclin-Dependent Kinase Inhibitor p16
0
Cyclin-Dependent Kinase Inhibitor p21
0
Eukaryotic Initiation Factor-2
0
Activating Transcription Factor 4
145891-90-3
Hydroxyl Radical
3352-57-6
Hydrogen
7YNJ3PO35Z
Hydrogen Peroxide
BBX060AN9V
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Scientific Research (C)
ID : 18K05537
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
EMBO J. 2001 Dec 3;20(23):6909-18
pubmed: 11726526
Subcell Biochem. 2002;36:19-37
pubmed: 12037982
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):344-6
pubmed: 12947735
Endocrinology. 2003 Oct;144(10):4264-71
pubmed: 12960105
Nat Med. 2007 Jun;13(6):688-94
pubmed: 17486089
J Biol Chem. 2008 Jul 4;283(27):18782-91
pubmed: 18450746
Biochem Biophys Res Commun. 2008 Dec 26;377(4):1195-8
pubmed: 18996093
Nat Prod Rep. 2008 Dec;25(6):1118-30
pubmed: 19030605
Neurosci Lett. 2009 Apr 3;453(2):81-5
pubmed: 19356598
Biochem Biophys Res Commun. 2009 Aug 21;386(2):316-21
pubmed: 19523450
J Clin Biochem Nutr. 2010 May;46(3):269-76
pubmed: 20490324
Oxid Med Cell Longev. 2011;2011:809696
pubmed: 21904647
Med Gas Res. 2012 Jan 24;2:1
pubmed: 22273079
Immunity. 2012 Mar 23;36(3):401-14
pubmed: 22342844
Med Gas Res. 2012 May 20;2(1):15
pubmed: 22608009
Cell Mol Life Sci. 2013 Oct;70(19):3493-511
pubmed: 23354059
ScientificWorldJournal. 2014 Jan 21;2014:417842
pubmed: 24634611
Prev Nutr Food Sci. 2014 Sep;19(3):129-35
pubmed: 25320709
Am J Physiol Cell Physiol. 2015 Apr 15;308(8):C621-30
pubmed: 25567807
Med Gas Res. 2015 Oct 26;5:13
pubmed: 26504515
Nature. 2016 Feb 11;530(7589):184-9
pubmed: 26840489
Circ J. 2016 Aug 25;80(9):2037-46
pubmed: 27477846
Biochem Biophys Res Commun. 2017 Jan 22;482(4):1183-1189
pubmed: 27923656
Rejuvenation Res. 2017 Aug;20(4):334-345
pubmed: 28285577
Sci Rep. 2017 Jun 15;7(1):3606
pubmed: 28620198
Biochem Biophys Res Commun. 2017 Oct 7;492(1):74-81
pubmed: 28807355
Curr Alzheimer Res. 2018 Mar 14;15(5):482-492
pubmed: 29110615
Nat Med. 2018 Aug;24(8):1246-1256
pubmed: 29988130
Biomed Pharmacother. 2018 Oct;106:1126-1134
pubmed: 30119179
Sci Rep. 2018 Nov 14;8(1):16822
pubmed: 30429524
Mol Cell Biochem. 1995 Jul 5;148(1):45-57
pubmed: 7476933