Large Timescale Interrogation of Neuronal Function by Fiberless Optogenetics Using Lanthanide Micro-particles.

Animals Behavior, Animal / drug effects Brain / metabolism Lanthanoid Series Elements / pharmacology Mice Mice, Transgenic Opsins / metabolism Optic Nerve / metabolism Optogenetics
C1V1 Optogenetics adeno-associated virus vector anion channelrhodopsin channelrhodopsin fiberless infrared lanthanide upconversion

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
22 01 2019
Historique:
received: 08 01 2018
revised: 08 10 2018
accepted: 27 12 2018
entrez: 24 1 2019
pubmed: 24 1 2019
medline: 7 3 2020
Statut: ppublish

Résumé

Optogenetics requires implantation of light-delivering optical fibers, as current light-sensitive opsins are activated by visible light, which cannot effectively penetrate biological tissues. Insertion of optical fibers and subsequent photostimulation inherently damages brain tissue, and fiber tethering can restrict animal behavior. To overcome these technical limitations, we developed minimally invasive "fiberless" optogenetics using lanthanide micro-particles (LMPs), which emit up-conversion luminescence in the visible spectrum in response to irradiation with tissue-penetrating near-infrared light. Depolarizing (C1V1) and hyperpolarizing (ACR1) opsins were strongly activated by up-conversion luminescence from green-emitting LMPs both in vitro and in vivo. Using this technique, we successfully manipulated locomotive behavior of mice by activating and inhibiting neurons in the dorsal striatum, at a depth of 2 mm from the brain surface. LMPs were retained and remained functional for >8 weeks at the injection site. Fiberless optogenetics offers opportunities to control neuronal function over longer time frames using freely behaving animals.

Identifiants

DOI: 10.1016/j.celrep.2019.01.001 PMID: 30673599
pubmed: 30673599
pii: S2211-1247(19)30001-4
doi: 10.1016/j.celrep.2019.01.001
pii:
doi:

Substances chimiques

Lanthanoid Series Elements 0
Opsins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Retracted Publication

Langues

eng

Sous-ensembles de citation

IM

Pagination

1033-1043.e5

Commentaires et corrections

Type : RetractionIn

Informations de copyright

Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

Auteurs

Toh Miyazaki (T)

Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan; CREST, JST, Honcho Kawaguchi, Saitama 332-0012, Japan.

Srikanta Chowdhury (S)

Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan; CREST, JST, Honcho Kawaguchi, Saitama 332-0012, Japan.

Takayuki Yamashita (T)

Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan; CREST, JST, Honcho Kawaguchi, Saitama 332-0012, Japan; PRESTO, JST, Honcho Kawaguchi, Saitama 332-0012, Japan.

Takanori Matsubara (T)

Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.

Hiromu Yawo (H)

Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai 980-8577, Japan.

Hideya Yuasa (H)

School of Life Science and Technology, Tokyo Institute of Technology Yokohama 226-8501, Japan.

Akihiro Yamanaka (A)

Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan; CREST, JST, Honcho Kawaguchi, Saitama 332-0012, Japan. Electronic address: yamank@riem.nagoya-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Comportement et mécanismes comportementaux › Comportement › Comportement animal › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Terres rares › Lanthanides › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Pigments rétiniens › Opsines › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Système nerveux › Système nerveux périphérique › Nerfs périphériques › Nerfs crâniens › Nerf optique › Large Timescale Interrogation of Neuronal...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Optogénétique › Large Timescale Interrogation of Neuronal...