Alveolar proteins stabilize cortical microtubules in Toxoplasma gondii.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 01 2019
23 01 2019
Historique:
received:
31
07
2018
accepted:
04
01
2019
entrez:
25
1
2019
pubmed:
25
1
2019
medline:
9
2
2019
Statut:
epublish
Résumé
Single-celled protists use elaborate cytoskeletal structures, including arrays of microtubules at the cell periphery, to maintain polarity and rigidity. The obligate intracellular parasite Toxoplasma gondii has unusually stable cortical microtubules beneath the alveoli, a network of flattened membrane vesicles that subtends the plasmalemma. However, anchoring of microtubules along alveolar membranes is not understood. Here, we show that GAPM1a, an integral membrane protein of the alveoli, plays a role in maintaining microtubule stability. Degradation of GAPM1a causes cortical microtubule disorganisation and subsequent depolymerisation. These changes in the cytoskeleton lead to parasites becoming shorter and rounder, which is accompanied by a decrease in cellular volume. Extended GAPM1a depletion leads to severe defects in division, reminiscent of the effect of disrupting other alveolar proteins. We suggest that GAPM proteins link the cortical microtubules to the alveoli and are required to maintain the shape and rigidity of apicomplexan zoites.
Identifiants
pubmed: 30674885
doi: 10.1038/s41467-019-08318-7
pii: 10.1038/s41467-019-08318-7
pmc: PMC6344517
doi:
Substances chimiques
Microtubule-Associated Proteins
0
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
401Subventions
Organisme : European Research Council
ID : 309255
Pays : International
Organisme : NIH HHS
ID : DP5 OD017892
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI123746
Pays : United States
Références
J Exp Med. 2007 Jun 11;204(6):1281-7
pubmed: 17562819
Cell Host Microbe. 2010 Oct 21;8(4):343-57
pubmed: 20951968
J Cell Sci. 2000 Apr;113 ( Pt 7):1241-54
pubmed: 10704375
Mol Biochem Parasitol. 2013 May;189(1-2):43-53
pubmed: 23685344
PLoS Pathog. 2010 Sep 09;6(9):e1001094
pubmed: 20844581
J Cell Sci. 2002 Mar 1;115(Pt 5):1017-25
pubmed: 11870220
Planta. 1990 Oct;182(3):363-9
pubmed: 24197186
Nat Methods. 2013 Feb;10(2):125-7
pubmed: 23263690
EMBO J. 2013 Jun 12;32(12):1702-16
pubmed: 23695356
PLoS One. 2017 Jun 22;12(6):e0179709
pubmed: 28640849
PLoS One. 2014 Jan 29;9(1):e85763
pubmed: 24489670
J Cell Biol. 2002 Mar 18;156(6):1039-50
pubmed: 11901169
Mol Biol Cell. 2004 Apr;15(4):1960-8
pubmed: 14742718
Tex Rep Biol Med. 1959;17:314-22
pubmed: 13818625
J Cell Biol. 2009 Jun 29;185(7):1135-48
pubmed: 19564400
Plant J. 2013 Jul;75(2):230-44
pubmed: 23398214
EMBO J. 2012 Dec 12;31(24):4524-34
pubmed: 23149386
Nat Commun. 2017 Nov 20;8(1):1613
pubmed: 29151572
J Cell Biol. 2004 May 10;165(3):383-93
pubmed: 15123738
Mol Biol Cell. 2007 Dec;18(12):4711-20
pubmed: 17881728
Cell Microbiol. 2017 Apr;19(4):
pubmed: 27696623
Sci Rep. 2017 Nov 14;7(1):15577
pubmed: 29138437
PLoS Pathog. 2016 Feb 04;12(2):e1005403
pubmed: 26845335
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
PLoS Pathog. 2017 Oct 6;13(10):e1006659
pubmed: 28985225
Cell Microbiol. 2010 Mar;12(3):362-71
pubmed: 19863555
PLoS Pathog. 2016 Jan 13;12(1):e1005388
pubmed: 26760042
J Cell Biol. 2015 Nov 23;211(4):757-63
pubmed: 26598613
J Integr Plant Biol. 2015 Apr;57(4):330-40
pubmed: 25693826
Nature. 2007 Apr 26;446(7139):1066-9
pubmed: 17460669
Eukaryot Cell. 2009 Apr;8(4):530-9
pubmed: 19218426
mBio. 2015 Sep 15;6(5):e00845-15
pubmed: 26374117
PLoS One. 2015 Jun 19;10(6):e0130356
pubmed: 26090798
Sci Rep. 2017 Feb 16;7:42746
pubmed: 28202940
Mol Biochem Parasitol. 1996 May;77(2):235-9
pubmed: 8813669
J Immunol. 1988 Nov 15;141(10):3584-91
pubmed: 3183382
PLoS One. 2014 Mar 14;9(3):e91819
pubmed: 24632839
Nat Methods. 2007 Dec;4(12):1003-5
pubmed: 17994029
J Protozool. 1987 May;34(2):217-26
pubmed: 3585817
Eukaryot Cell. 2012 Feb;11(2):206-16
pubmed: 22021240
PLoS Pathog. 2007 Aug 31;3(8):e115
pubmed: 17784785
mBio. 2015 Feb 17;6(1):e02357-14
pubmed: 25691595
J Biol Chem. 2009 Sep 11;284(37):25353-63
pubmed: 19561073
J Cell Sci. 1986 Feb;80:123-40
pubmed: 3722278
Cell. 2017 Jul 13;170(2):260-272.e8
pubmed: 28708996
Microbiol Mol Biol Rev. 2002 Mar;66(1):21-38; table of contents
pubmed: 11875126
Cell. 2016 Aug 25;166(5):1324-1337.e11
pubmed: 27565352
J Microsc. 2017 Aug;267(2):193-213
pubmed: 28328099
Eukaryot Cell. 2015 Jan;14(1):2-12
pubmed: 25380753
mSphere. 2017 Oct 18;2(5):
pubmed: 29062899
PLoS Pathog. 2017 May 5;13(5):e1006379
pubmed: 28475612
Mol Biol Cell. 2017 May 15;28(10):1361-1378
pubmed: 28331073
Eukaryot Cell. 2013 Dec;12(12):1588-99
pubmed: 23873863
Lab Chip. 2011 Feb 21;11(4):645-51
pubmed: 21180703
J Cell Sci. 2004 Nov 1;117(Pt 23):5697-705
pubmed: 15494366
Parasitol Res. 1994;80(2):91-8
pubmed: 8202461
PLoS One. 2014 Jun 27;9(6):e100450
pubmed: 24971596
J Cell Sci. 1997 Jan;110 ( Pt 1):35-42
pubmed: 9010782
Exp Parasitol. 1971 Oct;30(2):284-320
pubmed: 4399774
Elife. 2017 Mar 21;6:
pubmed: 28322189
Annu Rev Microbiol. 1999;53:629-55
pubmed: 10547703
PLoS One. 2011;6(8):e23977
pubmed: 21901148
J Cell Sci. 2015 Jun 15;128(12):2351-62
pubmed: 25964651
Nat Rev Microbiol. 2014 Feb;12(2):125-36
pubmed: 24384598
Cell. 2016 Sep 8;166(6):1423-1435.e12
pubmed: 27594426
Mol Biol Cell. 2016 Feb 1;27(3):549-71
pubmed: 26680740