Generation of induced pluripotent stem cells (IRMBi001-A) from an Alzheimer's disease patient carrying a G217D mutation in the PSEN1 gene.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
24
10
2018
revised:
19
12
2018
accepted:
31
12
2018
pubmed:
25
1
2019
medline:
7
8
2019
entrez:
25
1
2019
Statut:
ppublish
Résumé
Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 50 year-old patient suffering from Alzheimer's disease and carrying a G217D causal mutation on presenilin 1 (PSEN1). iPSCs were obtained following reprogramming using the integration-free Sendai Virus system which allows expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. iPS cells carry the patient G217D mutation and present a normal karyotype. The reported PS1-G217D iPSC line may be used to model and study human AD pathology in vitro.
Identifiants
pubmed: 30677723
pii: S1873-5061(18)30316-7
doi: 10.1016/j.scr.2018.101381
pii:
doi:
Substances chimiques
PSEN1 protein, human
0
Presenilin-1
0
Types de publication
Journal Article
Langues
eng
Pagination
101381Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.