CENP-A Ubiquitylation Contributes to Maintaining the Chromosomal Location of the Centromere.

CENP-A CUL4A-RBX1- COPS8 E3 ligase centromere centromere identity dimerization epigenetics kinetochore lysine 124 (K124), neocentromere ubiquitylation

Journal

Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009

Informations de publication

Date de publication:
22 Jan 2019
Historique:
received: 14 12 2018
revised: 15 01 2019
accepted: 20 01 2019
entrez: 26 1 2019
pubmed: 27 1 2019
medline: 24 4 2019
Statut: epublish

Résumé

The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)-RING-box protein 1 (RBX1)-COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.

Identifiants

pubmed: 30678315
pii: molecules24030402
doi: 10.3390/molecules24030402
pmc: PMC6384693
pii:
doi:

Substances chimiques

Centromere Protein A 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NCI NIH HHS
ID : R21 CA205659
Pays : United States

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Yohei Niikura (Y)

Greehey Children's Cancer Research Institute, Department of Molecular Medicine, UT Health Science Center San Antonio School of Medicine, 8403 Floyd Curl Drive, San Antonio, TX 78229-3000, USA. niikura@nicemice.cn.
MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing 210061, China. niikura@nicemice.cn.

Risa Kitagawa (R)

Greehey Children's Cancer Research Institute, Department of Molecular Medicine, UT Health Science Center San Antonio School of Medicine, 8403 Floyd Curl Drive, San Antonio, TX 78229-3000, USA. kitagawaR@uthscsa.edu.

Katsumi Kitagawa (K)

Greehey Children's Cancer Research Institute, Department of Molecular Medicine, UT Health Science Center San Antonio School of Medicine, 8403 Floyd Curl Drive, San Antonio, TX 78229-3000, USA. kitagawaK@uthscsa.edu.

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Classifications MeSH