Biological Performance of Electrospun Polymer Fibres.
aligned fibrous scaffolds
cell biology
electrospinning
nanofibers
Journal
Materials (Basel, Switzerland)
ISSN: 1996-1944
Titre abrégé: Materials (Basel)
Pays: Switzerland
ID NLM: 101555929
Informations de publication
Date de publication:
24 Jan 2019
24 Jan 2019
Historique:
received:
30
11
2018
revised:
21
01
2019
accepted:
22
01
2019
entrez:
27
1
2019
pubmed:
27
1
2019
medline:
27
1
2019
Statut:
epublish
Résumé
The evaluation of biological responses to polymeric scaffolds are important, given that the ideal scaffold should be biocompatible, biodegradable, promote cell adhesion and aid cell proliferation. The primary goal of this research was to measure the biological responses of cells against various polymeric and collagen electrospun scaffolds (polycaprolactone (PCL) and polylactic acid (PLA) polymers: PCL⁻drug, PCL⁻collagen⁻drug, PLA⁻drug and PLA⁻collagen⁻drug); cell proliferation was measured with a cell adhesion assay and cell viability using 5-bromo-2'-deoxyuridine (BrdU) and resazurin assays. The results demonstrated that there is a distinct lack of growth of cells against any irgasan (IRG) loaded scaffolds and far greater adhesion of cells against levofloxacin (LEVO) loaded scaffolds. Fourteen-day studies revealed a significant increase in cell growth after a 7-day period. The addition of collagen in the formulations did not promote greater cell adhesion. Cell viability studies revealed the levels of IRG used in scaffolds were toxic to cells, with the concentration used 475 times higher than the EC
Identifiants
pubmed: 30682805
pii: ma12030363
doi: 10.3390/ma12030363
pmc: PMC6384992
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Engineering and Physical Sciences Research Council
ID : EP/F50036X/1
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