A Multiparameter Flow Cytometry Analysis Panel to Assess CD163 mRNA and Protein in Monocyte and Macrophage Populations in Hyperinflammatory Diseases.

Antigens, CD / analysis Antigens, Differentiation, Myelomonocytic / analysis Cells, Cultured Flow Cytometry Humans Inflammation / immunology Macrophages / immunology Monocytes / immunology RNA, Messenger / analysis Receptors, Cell Surface / analysis

Journal

Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R

Informations de publication

Date de publication:
01 03 2019
Historique:
received: 01 06 2018
accepted: 22 12 2018
pubmed: 27 1 2019
medline: 13 11 2019
entrez: 27 1 2019
Statut: ppublish

Résumé

CD163 facilitates regulation and resolution of inflammation and removal of free hemoglobin and is highly expressed in myeloid cells from patients with inflammatory disorders, such as systemic juvenile idiopathic arthritis (SJIA) and macrophage activation syndrome (MAS). Our recent studies indicate that regulation of CD163 mRNA expression is a key functional property of polarized monocytes and macrophages and is mediated at the transcriptional and posttranscriptional level, including via microRNAs. The goal of the current study is to develop a multiparameter flow cytometry panel incorporating detection of CD163 mRNA for polarized monocyte and macrophage populations in disorders such as SJIA and MAS. THP-1 cells and CD14

Identifiants

DOI: 10.4049/jimmunol.1800765 PMID: 30683706 PMC: PMC6382590
pubmed: 30683706
pii: jimmunol.1800765
doi: 10.4049/jimmunol.1800765
pmc: PMC6382590
mid: NIHMS1517661
doi:

Substances chimiques

Antigens, CD 0
Antigens, Differentiation, Myelomonocytic 0
CD163 antigen 0
RNA, Messenger 0
Receptors, Cell Surface 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1635-1643

Subventions

Organisme : NIAMS NIH HHS
ID : K08 AR072075
Pays : United States
Organisme : NIAMS NIH HHS
ID : P30 AR070549
Pays : United States

Informations de copyright

Copyright © 2019 by The American Association of Immunologists, Inc.

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Auteurs

Sherry Thornton (S)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.
Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45267.

Rachel Tan (R)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.
Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45267.

Alyssa Sproles (A)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.

Thuy Do (T)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.

Jonathan Schick (J)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.

Alexei A Grom (AA)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.
Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45267.

Monica DeLay (M)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and.

Grant S Schulert (GS)

Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229; and Grant.schulert@cchmc.org.
Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45267.
ORCID: 0000-0001-5923-7051

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Classifications MeSH

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