Characterization of recombinant fructose-1,6-bisphosphatase gene mutations: evidence of inhibition/activation of FBPase protein by gene mutation.
allosteric regulation
enzymatic activity
hyperglycemia
hypoglycemia
mutagenesis
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
28 02 2019
28 02 2019
Historique:
received:
15
06
2018
revised:
10
01
2019
accepted:
14
01
2019
pubmed:
27
1
2019
medline:
29
1
2020
entrez:
27
1
2019
Statut:
epublish
Résumé
Specific residues of the highly regulated fructose-1,6-bisphosphatase (FBPase) enzyme serve as important contributors to the catalytic activity of the enzyme. Previous clinical studies exploring the genetic basis of hypoglycemia revealed two significant mutations in the coding region of the FBPase gene in patients with hypoglycemia, linking the AMP-binding site to the active site of the enzyme. In the present study, a full kinetic analysis of similar mutants was performed. Kinetic results of mutants Y164A and M177A revealed an approximate two to three-fold decrease in inhibitory constants (
Identifiants
pubmed: 30683805
pii: BSR20180960
doi: 10.1042/BSR20180960
pmc: PMC6386767
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Adenosine Monophosphate
415SHH325A
Fructose-Bisphosphatase
EC 3.1.3.11
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 The Author(s).
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