Genetic Analysis of Protein Translocation.
Gene fusion
Prl mutant
Protein secretion
Protein translocation
Sec
Journal
The protein journal
ISSN: 1875-8355
Titre abrégé: Protein J
Pays: Netherlands
ID NLM: 101212092
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
pubmed:
27
1
2019
medline:
30
1
2020
entrez:
27
1
2019
Statut:
ppublish
Résumé
Cells in all domains of life must translocate newly synthesized proteins both across membranes and into membranes. In eukaryotes, proteins are translocated into the lumen of the ER or the ER membrane. In prokaryotes, proteins are translocated into the cytoplasmic membrane or through the membrane into the periplasm for Gram-negative bacteria or the extracellular space for Gram-positive bacteria. Much of what we know about protein translocation was learned through genetic selections and screens utilizing lacZ gene fusions in Escherichia coli. This review covers the basic principles of protein translocation and how they were discovered and developed. In particular, we discuss how lacZ gene fusions and the phenotypes conferred were exploited to identify the genes involved in protein translocation and provide insights into their mechanisms of action. These approaches, which allowed the elucidation of processes that are conserved throughout the domains of life, illustrate the power of seemingly simple experiments.
Identifiants
pubmed: 30684070
doi: 10.1007/s10930-019-09813-y
pii: 10.1007/s10930-019-09813-y
pmc: PMC6589372
mid: NIHMS1519641
doi:
Substances chimiques
Escherichia coli Proteins
0
Recombinant Fusion Proteins
0
SEC Translocation Channels
0
beta-Galactosidase
EC 3.2.1.23
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
217-228Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM118024
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35GM118024
Pays : United States
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