TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment.
Cell Differentiation
Cell Lineage
Chromatin
/ metabolism
Ectoderm
/ cytology
Epidermis
/ metabolism
Epigenesis, Genetic
Feedback, Physiological
Gene Regulatory Networks
Humans
Keratinocytes
/ cytology
Transcription Factor AP-2
/ metabolism
Transcription Factors
/ metabolism
Transcriptome
/ genetics
Tumor Suppressor Proteins
/ metabolism
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
07 02 2019
07 02 2019
Historique:
received:
22
03
2018
revised:
04
10
2018
accepted:
17
12
2018
pubmed:
29
1
2019
medline:
28
3
2020
entrez:
29
1
2019
Statut:
ppublish
Résumé
Tissue development results from lineage-specific transcription factors (TFs) programming a dynamic chromatin landscape through progressive cell fate transitions. Here, we define epigenomic landscape during epidermal differentiation of human pluripotent stem cells (PSCs) and create inference networks that integrate gene expression, chromatin accessibility, and TF binding to define regulatory mechanisms during keratinocyte specification. We found two critical chromatin networks during surface ectoderm initiation and keratinocyte maturation, which are driven by TFAP2C and p63, respectively. Consistently, TFAP2C, but not p63, is sufficient to initiate surface ectoderm differentiation, and TFAP2C-initiated progenitor cells are capable of maturing into functional keratinocytes. Mechanistically, TFAP2C primes the surface ectoderm chromatin landscape and induces p63 expression and binding sites, thus allowing maturation factor p63 to positively autoregulate its own expression and close a subset of the TFAP2C-initiated surface ectoderm program. Our work provides a general framework to infer TF networks controlling chromatin transitions that will facilitate future regenerative medicine advances.
Identifiants
pubmed: 30686763
pii: S1934-5909(18)30602-7
doi: 10.1016/j.stem.2018.12.012
pmc: PMC7135956
mid: NIHMS1035203
pii:
doi:
Substances chimiques
Chromatin
0
TFAP2C protein, human
0
TP63 protein, human
0
Transcription Factor AP-2
0
Transcription Factors
0
Tumor Suppressor Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
271-284.e8Subventions
Organisme : NIAMS NIH HHS
ID : F32 AR070565
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM109836
Pays : United States
Organisme : NCI NIH HHS
ID : P20 CA096470
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NHGRI NIH HHS
ID : P50 HG007735
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR073170
Pays : United States
Informations de copyright
Copyright © 2018. Published by Elsevier Inc.
Références
EMBO J. 2014 Feb 18;33(4):312-26
pubmed: 24451200
Cell. 2014 Dec 18;159(7):1665-80
pubmed: 25497547
Nat Methods. 2016 Nov;13(11):919-922
pubmed: 27643841
Nature. 1999 Apr 22;398(6729):708-13
pubmed: 10227293
Proc Natl Acad Sci U S A. 2017 Jun 20;114(25):E4914-E4923
pubmed: 28576882
PLoS One. 2008;3(10):e3441
pubmed: 18927616
Sci Transl Med. 2014 Nov 26;6(264):264ra163
pubmed: 25429056
Nature. 2012 Sep 6;489(7414):75-82
pubmed: 22955617
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Mol Cell Biol. 2012 May;32(9):1633-44
pubmed: 22371483
Development. 2002 Jun;129(11):2733-47
pubmed: 12015300
Nat Biotechnol. 2010 May;28(5):495-501
pubmed: 20436461
Annu Rev Pathol. 2010;5:349-71
pubmed: 20078223
Stem Cells. 2008 Feb;26(2):372-80
pubmed: 17962700
Development. 2013 Nov;140(22):4533-43
pubmed: 24131634
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
Am J Hum Genet. 2012 Sep 7;91(3):435-43
pubmed: 22922031
Cell Res. 2012 Nov;22(11):1546-61
pubmed: 22945355
Nucleic Acids Res. 2015 Jan;43(2):862-74
pubmed: 25567987
Nat Biotechnol. 2014 Feb;32(2):171-178
pubmed: 24441470
Nature. 2015 Feb 12;518(7538):249-53
pubmed: 25470060
Nucleic Acids Res. 2007;35(11):3705-12
pubmed: 17517782
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Nature. 1999 Apr 22;398(6729):714-8
pubmed: 10227294
Nat Methods. 2013 Dec;10(12):1213-8
pubmed: 24097267
Genes Chromosomes Cancer. 2010 Oct;49(10):948-62
pubmed: 20629094
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Nucleic Acids Res. 2012 Aug;40(15):7190-206
pubmed: 22573176
Annu Rev Cell Dev Biol. 2007;23:93-113
pubmed: 17489688
Nat Genet. 2018 Dec;50(12):1658-1665
pubmed: 30397335
Sci Transl Med. 2014 Nov 26;6(264):264ra164
pubmed: 25429057
Sci Transl Med. 2014 Nov 26;6(264):264ra165
pubmed: 25429058
Sci Rep. 2016 Aug 23;6:32007
pubmed: 27550649
Stem Cells Int. 2012;2012:564612
pubmed: 22550511
J Biol Chem. 2003 May 2;278(18):16021-9
pubmed: 12586840