Amoebicidal and Amoebistatic Effects of Artemisia argyi Methanolic Extracts on Acanthamoeba castellanii Trophozoites and Cysts.
Acanthamoeba castellanii
Amoebicidal activity
Antioxidant activity
Artemisia argyi
Cytotoxic potential
Phytotoxic activity
Journal
Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
07
07
2018
accepted:
23
11
2018
pubmed:
29
1
2019
medline:
23
7
2019
entrez:
29
1
2019
Statut:
ppublish
Résumé
PURPOSE : The present study aimed to investigate the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and on cysts. We also determined cytotoxic effect, enzymatic and non-enzymatic antioxidant activities, total phenolic, lavonoid and antioxidative contents of A. argyi. A. argyi was harvested from various geographic sites in Ordu province in Turkey. The fresh leaves were subjected to methanolic extraction. In 100 μl culture, different concentrations of A. argyi methanolic extract (in quantities from 1.2, 2.3, 4.7, 9.4, 18.7, 37.4, 74.8 mg/ml) and the same volume of trophozoite/cyst suspension were mixed for the determination of the amoebicidal activity of the plant extract. Human bronchial epithelial cells were treated with the same concentrations of Artemisia extracts to determine cytotoxic potential. Total phenolic and lavonoid contents of the extract were calculated as 261 mg gallic acid/g dry extract and 29 mg quercetin/g dry extract, respectively. Total antioxidant activity was also calculated as 367 mg ascorbic acid/g dry extract. The growth of trophozoites stopped in A. argyi methanolic extract with 50% inhibitory concentrations (IC50)/8 h for 37.4 mg/ ml and 74.8 mg/ml extract solution and had stronger amoebicidal activity on the cysts with IC50/72 h. Artemisia showed stronger inhibitory effects on bronchial epithelial cells at the concentrations of 9.4, 18.7, 37.4 and 74.8 mg/ml. The study indicated that A. argyi leaf extract has cytotoxic and anti-amoebic activities.
Identifiants
pubmed: 30689190
doi: 10.2478/s11686-018-00009-5
pii: 10.2478/s11686-018-00009-5
doi:
Substances chimiques
Amebicides
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
63-70Références
Akin Polat Z.,Vural A.,Tepe B., Cetin A. 2007. In vitro amoebicidal activity of four Allium species on Acanthamoeba castellani and their cytotoxic potentials on corneal cells. Parasitology Research, 101, 397–402. https://doi.org/10.1007/s00436-007-0487-x
doi: 10.1007/s00436-007-0487-x
Arvouet-Grand A., Vennat B., Pourrat A., Legret P. 1994. Standardization of propolis extract and identification of principal constituents. Journal DePharmacie De Belgique, 49, 462–468. PMID:7884635
Badria F.A., Hetta M.H., Sarhan R.M., Ezz El-Din M.H. 2014. Lethal effects of Helianthemum lippii (L.) on Acanthamoeba castellanii cysts in vitro. Korean Journal Parasitology, 52(3), 243–9. https://doi.org/10.3347/kjp.2014.52.3.243
doi: 10.3347/kjp.2014.52.3.243
Barney J.N., DiTommase A. 2003.The biology of Canadian weeds. 118. Artemisia vulgaris L. Canadian Journal of Plant Science, 83, 205–215. https://doi.org/10.4141/p01-098
doi: 10.4141/P01-098
Beauchamp C., Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276–287.
doi: 10.1016/0003-2697(71)90370-8
Benzie I.F.F., Strain J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239,70–77. https://doi.org/10.1006/abio.1996.0292
doi: 10.1006/abio.1996.0292
pubmed: 8660627
Carvalho I.S., Cavaco T., Brodelius M. 2011. Phenolic composition and antioxidant capacity of six Artemisia species. Industrial Crops and Products, 33, 382–388. https://doi.org/10.1016/j.indcrop.2010.11.005
doi: 10.1016/j.indcrop.2010.11.005
Chevallier A. (Ed.). 1996. The encyclopedia of medicinal plants. Dorling Kindersley, London.
Degerli S., Berk S., Malatyali E., Tepe B. 2012a. Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch & C.A. Mey) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites. Parasitology Research, 110, 565–570. https://doi.org/10.1007/s00436-011-2524-z
doi: 10.1007/s00436-011-2524-z
pubmed: 21735149
Degerli S., Tepe B., Celiksoz A., Berk S., Malatyali E. 2012b. In vitro amoebicidal activity of Origanum syriacum and Origanumla evigatum on Acanthamoeba castellanii cysts and trophozoites. Experimental Parasitology, 131, 20–24. https://doi.org/10.1016/j.exppara.2012.02.020
doi: 10.1016/j.exppara.2012.02.020
pubmed: 22417972
Derda M., Hadaś E.,Thiem B. 2009. Plant extracts as natural amoebicidal agents. Parasitology Research, 104(3),705–708. https://doi.org/10.1007/s00436-008-1277-9
doi: 10.1007/s00436-008-1277-9
pubmed: 19050923
Derda M., Hadaś E., Cholewiński M., Skrzypczak Ł., Grzondziel A., Wojtkowiak-Giera A. 2016. Artemisia annua L. as a plant with potential use in the treatment of acanthamoebiasis. Parasitology Research, 115(4), 1635–1639. https://doi.org/10.1007/s00436-016-4902-z
doi: 10.1007/s00436-016-4902-z
pubmed: 26782959
pmcid: 4799240
Dinis T.C.P., Madeira V.M.C., Almeida L.M. 1994. Action of phenolic derivatives (Acetaminophen, Salicylate, and 5-Aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315, 161–169. https://doi.org/10.1006/abbi.1994.1485
doi: 10.1006/abbi.1994.1485
pubmed: 7979394
El-Sayed N.M., Ismail K.A., Ahmed S.A., Hetta M.H. 2012. In vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L., Curcuma longa L. and Pancratium maritimum L. on Acanthamoeba castellanii cysts. Parasitology Research, 110(5), 1985–1992
doi: 10.1007/s00436-011-2727-3
Farnsworth N. R., Bunyapraphatsara N. (Ed.). 1992. Thai medicinal plants: recommended for primary health care system. Medicinal Plant Information Center, Bangkok, Thailand, pp.409
Firestone G.L., Sundar S.N. 2009. Anticancer activities of artemisinin and its bioactive derivatives. Expert Reviews in Molecular Medicine, 11, e32. https://doi.org/10.1017/s1462399409001239
doi: 10.1017/S1462399409001239
pubmed: 19883518
Fu R., Zhang Y., Guo Y., Liu F., Chen F. 2014. Determination of phenolic contents and antioxidant activities of extracts of Jatropha curcas L. seed shell, a by-product, a new source of natural antioxidant. Industrial Crops and Products, 58, 265–70. https://doi.org/10.1016/j.indcrop.2014.04.031
doi: 10.1016/j.indcrop.2014.04.031
Ge Y. B., Wang Z. G., Xiong Y., Huang X.J., Mei Z.N. 2016. Anti-inflammatory and blood stasis activities of essential oil extracted from Artemisia argyi leaf in animals. Journal of Natural Medicines, 70 (3), 531–538. https://doi.org/10.1007/s11418-016-0972-6
doi: 10.1007/s11418-016-0972-6
pubmed: 26894818
Gordanian B., Behbahani M., Carapetian J., Fazilati M. 2014. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species. Research in Pharmaceutical Sciences, 9 (2),91–96. PMID: 25657777.
pubmed: 25657777
pmcid: 4311295
Huang H.C., Wang H.F., Yih K.H., Chang L.Z., Chang T.M. 2012. Dual bioactivities of essential oil extracted from the leaves of Artemisia argyi as an antimelanogenic versus antioxidant agent and chemical composition analysis by GC/MS. International Journal of Molecular Sciences, 13, 14679–14697. https://doi.org/10.3390/ijms131114679
doi: 10.3390/ijms131114679
pubmed: 23203088
pmcid: 3509604
Kanatt S. R., Chawla S.P., Sharma A. 2014. Antioxidant and radio-protective activities of lemon grass and staranise extracts. Food Bioscience, 6 (24), 24–30. https://doi.org/10.1016/j.fbio.2014.03.002
doi: 10.1016/j.fbio.2014.03.002
Kao P.M., Hsu B.M., Chen N.H., Huang K.H., Huang S.W., King K.L., Chiu Y.C. 2012. Isolation and identification of Acanthamoeba species from thermal spring environments in southern Taiwan. Experimental Parasitology, 130(4), 354–358. https://doi.org/10.1016/j.exppara.2012.02.008
doi: 10.1016/j.exppara.2012.02.008
pubmed: 22366362
Karimi A., Hadian J., Farzaneh M., Khadivi-Khub A. 2015. Evaluation of genetic variability, rust resistance and marker-detection in cultivated Artemisia dracunculus from Iran. Gene, 554(2), 224–232. https://doi.org/10.1016/j.gene.2014.10.057
doi: 10.1016/j.gene.2014.10.057
pubmed: 25445292
Keyhani J., Keyhani E. Anti-oxidative stress enyzmes in golden chanterelle (Cantharellus cibarius), 2012. In: (Eds A. Mendez-Vilas), Microbes in Applied Research. Singapore: World Scientific, pp. 23–27.
doi: 10.1142/9789814405041_0005
Khan N.A. (Ed.). 2009. Acanthamoeba biology and pathogenesis, Caister Academic Press, Norfolk, UK, pp. 85.
Kim J.K., Shin E.C., Lim H.J., Choi S.J., Kim C.R., Suh S.H., 2015. Characterization of nutritional composition, antioxidative capacity, and sensory attributes of seomaemug wort, a native korean variety of Artemisia argyi H. Lév. & Vaniot. Journal of Analytical Methods in Chemistry, 1–9. https://doi.org/10.1155/2015/916346
Kitagawa K., Nakamura T., Takahashi N., Oikawa Y., Ikeda T. 2003. A novel combination treatment of chlorohexidine gluconate, natamycin (pimaricin) and debridement for a Acanthamoeba keratitis. Japanese Journal of Ophthalmology, 47, 616–617. https://doi.org/10.1016/j.jjo.2003.08.005
doi: 10.1016/j.jjo.2003.08.005
pubmed: 14636856
Koloren O., Koloren Z., Eker S. 2016. Molecular phylogeny of Artemisia species based on internal transcribed spacer (ITS) of 18S-26S rDNA in Ordu Province of Turkey. Biotechnology & Biotechnological Equipment, 30, 929–934. https://doi.org/10.1371/journal.pone.0197458
doi: 10.1371/journal.pone.0197458
Lee J. H., Lee J.M., Lee S.H., Kim Y.G., Lee S., Kim S. et al. 2015. Comparison of artemisin in content and antioxidant activity from various organs of Artemisia species. Horticulture Environment and Biotechnology, 56, 697–703. https://doi.org/10.1007/s13580-015-0143-9
doi: 10.1007/s13580-015-0143-9
Magana M.L., Fernandez-Diez J., Magana M. 2008. Cutaneous amebiasis in pediatrics. Archives of Dermatology, 144(10), 1369–72. https://doi.org/10.1001/archderm.144.10.1369
doi: 10.1001/archderm.144.10.1369
pubmed: 18936402
Malatyali E., Tepe B., Değerli S., Berk S., Akpulat H.A. 2012. In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites. Parasitology Research, 110, 167–174. https://doi.org/10.1007/s00436-011-2466-5 .
doi: 10.1007/s00436-011-2466-5
pubmed: 21626154
Morrison A.O., Morris R., Shannon A., Lauer S.R., Guarner J., Kraft C.S. 2016. Disseminated Acanthamoeba Infection Presenting With Cutaneous Lesions in an Immunocompromised Patient: A Case Report, Review of Histomorphologic Findings, and Potential Diagnostic Pitfalls. American Journal of Clinical Pathology, 145(2), 266–70. https://doi.org/10.1093/ajcp/aqv081 .
doi: 10.1093/ajcp/aqv081
pubmed: 26800765
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods,65 (1–2), 55–63. https://doi.org/10.1016/0022-1759(83)90303-4
doi: 10.1016/0022-1759(83)90303-4
pubmed: 6606682
Nakisah M.A., Ida Muryany M.Y., Fatimah H., Nor Fadilah R., Zalilawati M.R., Khamsah S. 2012. Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii. World Journal of Microbiology & Biotechnology, 28, 1237–1244. https://doi.org/10.1007/s11274-011-0927-8
doi: 10.1007/s11274-011-0927-8
Nawab A., Yunus M., Mahdi A., Gupta S. 2011. Evaluation of anticancer properties of medicinal plants from the Indian sub-continent. Molecular and Cellular Pharmacology, 3, 21–29. https://doi.org/10.4255/mcpharmacol.11.04
doi: 10.4255/mcpharmacol.11.04
Oliaee D., Boroushaki T.M., Oliaee N., Ghorbani A. 2014. Evaluation of cytotoxicity and antifertility effect of Artemisia kopetdaghensis. Advances in Pharmaceutical Sciences, 5 pages. https://doi.org/10.1155/2014/745760 .
doi: 10.1155/2014/745760
Oyaizu M. 1986. Studies on products of browning reaction-antioxidative activities of products of browning reaction prepared from glucosamine. Journal of Nutrition, 44,307–315. https://doi.org/10.5264/eiyogakuzashi.44.307
doi: 10.5264/eiyogakuzashi.44.307
Piao G.C., Li Y.X., Yuan H.D., Jin G.Z. 2012. Cytotoxic fraction from Artemisia sacrorum against three human cancer cell lines and separation and identification of its compounds. Natural Product Research, 26 (16), 1483–1491. https://doi.org/10.1080/14786419.2011.565473
doi: 10.1080/14786419.2011.565473
pubmed: 22008023
Saleh A.M., Aljada A., Rizvi S.A., Nasr A., Alaskar A.S., Williams J.D. 2014. In vitro cytotoxicity of Artemisia vulgaris L. essential oil is mediated by a mitochondria-dependent apoptosis in HL-60 leukemic cell line. BMC Complementary and Alternative Medicine, 14, 226. https://doi.org/10.1186/1472-6882-14-226 .
doi: 10.1186/1472-6882-14-226
pubmed: 25002129
pmcid: 4227289
Sanchez-Moreno C., Larrauri J. A., Saura-Calixto F. 1998. A procedure to measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture, 76, 270–276. https://doi.org/10.1002/(sici)1097-0010(199802)76
doi: 10.1002/(SICI)1097-0010(199802)76
Santos I.G, Scher R., Rott M.B., Menezes L.R., Costa E.V., Cavalcanti S.C. 2016. Amebicidal activity of the essential oils of Lippia spp. (Verbenaceae) against Acanthamoeba polyphaga trophozoites. Parasitology Research, 115(2), 535–40. https://doi.org/10.1007/s00436-015-4769-4
doi: 10.1007/s00436-015-4769-4
pubmed: 26446087
Sarath V.J., So C.S., Young D.W., Gollapudi S. 2007. Artemisia princeps var. orientalis induces apoptosis in human breast cancer MCF-7 cells. Anticancer Research, 27, 3891–3898. PMID:18225547
pubmed: 18225547
Schuster F.L, Visvesvara G.S. 2004. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. International Journal of Parasitology, 34, 1001–1027. https://doi.org/10.1016/j.ijpara.2004.06.004
doi: 10.1016/j.ijpara.2004.06.004
pubmed: 15313128
Seal D.V. 2003. Acanthamoeba keratitis update-incidence, molecular epidemiology and new drugs for treatment. Eye, 17, 893–905. https://doi.org/10.1038/sj.eye.6700563
doi: 10.1038/sj.eye.6700563
Seo H.J., Park K.K., Han S.S., Chung W.Y., Son M.W., Kim W.B., Surh Y.J. 2002. Inhibitory effects of the standardized extract (DA-9601) of Artemisia asiatica Nakaionphorbol ester-induced ornithine decarboxylase activity, papilloma formation, cyclooxygenase-2 expression, inducible nitric oxide synthase expression and nuclear transcription factor kappa B activation in mouse skin. International Journal of Cancer, 100 (4), 456–462. https://doi.org/10.1002/ijc.10489
doi: 10.1002/ijc.10489
pubmed: 12115530
Sharmila K., Padma P.R. 2013. Anticancer activity of Artemisia vulgaris on hepatocellular carcinoma (HepG2) cells. International Journal of Pharmaceutical Sciences, 5 (3), 479–483.
Shoaib H.M, Muazzam A.G, Mir A, Jung S.Y, Matin A. 2013. Evaluation of inhibitory potential of some selective methanolic plants extracts on biological characteristics of Acanthamoeba castellanii using human corneal epithelial cells in vitro. Parasitology Research, 112(3),1179–88. https://doi.org/10.1007/s00436-012-3249-3
doi: 10.1007/s00436-012-3249-3
pubmed: 23306385
Sifaoui I., López-Arencibia A., Martín-Navarro C.M., Chammem N., Mejri M., Lorenzo-Morales J., Abderabba M., Piñero J.E. 2013. Activity assessment of Tunisian olive leaf extracts against the trophozoite stage of Acanthamoeba. Parasitology Research, 112, 2825–2829. https://doi.org/10.1007/s00436-013-3453-9 .
doi: 10.1007/s00436-013-3453-9
pubmed: 23681194
Silva L., Pezzini B.R., Soares L. 2015. Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves. Pharmacognosy Magazine, 11, 96–101. https://doi.org/10.4103/0973-1296.149721
doi: 10.4103/0973-1296.149721
pubmed: 25709217
pmcid: 4329640
Singleton V.L., Rossi J.A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16,144–158.
Sun L., Zhang J., Lu X., Zhang L., Zhang Y. 2011. Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves. Food and Chemical Toxicology, 49 (10), 2689–2696. https://doi.org/10.1016/j.fct.2011.07.042 .
doi: 10.1016/j.fct.2011.07.042
pubmed: 21802475
Taherkhani M. 2015. Chemical constituents and in vitro anticancer, cytotoxic, mutagenic and antimutagenic activities of Artemisia diffusa. Pharmaceutical Chemistry Journal, 48 (11), 727–732. https://doi.org/10.1007/s11094-015-1182-3
doi: 10.1007/s11094-015-1182-3
Taherkhani M. 2017. Chemical constituents, total phenolic content, antimicrobial, antioxidant and radical scavenging properties, chelating ability, tyrosinase inhibition and in vitro cytotoxic effects of Artemisia aucheri Herbs. Pharmaceutical Chemistry Journal, 50 (11), 736–745. https://doi.org/10.1007/s11094-017-1523-5
doi: 10.1007/s11094-017-1523-5
Tepe B., Malatyali E., Degerli S., Berk S. 2012. In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba castellanii trophozoites and cysts. Parasitology Research, 110 (5),1773–1778. https://doi.org/10.1007/s00436-011-2698-4 .
doi: 10.1007/s00436-011-2698-4
pubmed: 22037826
Wanachiwanawin D., Booranapong W., Kosrirukvongs P. 2012. Clinical features of Acanthamoeba keratitis in contact lens wearers and nonwearers. Southeast Asian J Trop Med Public Health, 43(3), 549–556.
pubmed: 23077833
Watson L.E., Bates P.L., Evans T.M., Unwin M.M., Estes J.R. 2002. Molecular phylogeny of subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. BMC Evolutionary Biology, 2,17. https://doi.org/10.1186/1471-2148-2-17 .
doi: 10.1186/1471-2148-2-17
pubmed: 12350234
pmcid: 130036
Yoder J.S., Verani J., Heidman N., Hoppe-Bauer J., Alfonso E.C., Miller D., Jones D.B., Bruckner D., Langston R., Jeng B.H., Joslin C.E., Tu E., Colby K., Vetter E., Ritterband D., Mathers W., Kowalski R.P., Acharya N.R., Limaye AP., Leiter C., Roy S., Lorick S., Roberts J., Beach M.J. 2012. Acanthamoeba keratitis: the persistence of cases following a multistate outbreak. Ophthalmic Epidemiology, 19(4), 221–225. https://doi.org/10.3109/09286586.2012.681336
doi: 10.3109/09286586.2012.681336
pubmed: 22775278
Zhang L.B., Lv J.L., Chen H.L., Yan X.Q., Duan J.A. 2013. Chemical constituents from Artemisia argyi and their chemotaxonomic significance. Biochemical Systematics and Ecology, 50,455–458. https://doi.org/10.1016/j.bse.2013.06.010 .
doi: 10.1016/j.bse.2013.06.010
Zhao F., Shi B., Sun D., Chen H., Tong M., Zhang P. 2016. Effects of dietary supplementation of Artemisia argyi aqueous extract on antioxidant indexes of small intestine in broilers. Animal Nutrition, 2 (3),198–203. https://doi.org/10.1016/j.aninu.2016.06.006
doi: 10.1016/j.aninu.2016.06.006
pubmed: 29767092
pmcid: 5941037