Presence of the point mutations Val1016Gly in the voltage-gated sodium channel detected in a single mosquito from Panama.
Aedes
/ classification
Amino Acid Substitution
Animals
Chikungunya Fever
/ transmission
Dengue
/ transmission
Drug Resistance
Female
Insecticides
/ pharmacology
Male
Mosquito Vectors
/ drug effects
Panama
/ epidemiology
Point Mutation
Pyrethrins
/ pharmacology
Voltage-Gated Sodium Channels
/ genetics
Zika Virus Infection
/ transmission
Aedes aegypti
Aedes albopictus
Panama
Pyrethroid resistance
Voltage-gated sodium channel
kdr mutations
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
28 Jan 2019
28 Jan 2019
Historique:
received:
04
06
2018
accepted:
10
01
2019
entrez:
30
1
2019
pubmed:
30
1
2019
medline:
5
2
2019
Statut:
epublish
Résumé
In Panama, arboviroses such as dengue fever, and more recently chikungunya fever and Zika disease, are transmitted by Aedes aegypti and Aedes albopictus. Their control is based on the elimination of breeding sites and fogging with pyrethroid insecticides. However, one of the significant issues derived from the prolonged use of pyrethroid insecticide is the development of resistance mechanisms, such as knockdown resistance or kdr. The objective of this study was to evaluate the presence of kdr mutations in a partial region of the VGSC gene in samples of wild-caught Aedes mosquitoes from different locations of the Metropolitan Region of Panama. Based on the analysis of 194 sequences of the VGSC gene, two kdr mutations (Ile1011Met and Val1016Gly) were detected in a specimen of Ae. aegypti. The frequency of kdr mutations in the evaluated samples of Ae. aegypti was 0.01. This study provides evidence for a low frequency of kdr mutations in Ae. aegypti populations in Panama. It is possible that these changes have no impact on vector control interventions. To our knowledge, we report, for the first time in America the Val1016Gly mutation documented in Asia. In general terms, this result is highly relevant to the Aedes Control Programme in Panama since it constitutes a feasible approach for the timely detection of resistance as well as for the development of strategies.
Sections du résumé
BACKGROUND
BACKGROUND
In Panama, arboviroses such as dengue fever, and more recently chikungunya fever and Zika disease, are transmitted by Aedes aegypti and Aedes albopictus. Their control is based on the elimination of breeding sites and fogging with pyrethroid insecticides. However, one of the significant issues derived from the prolonged use of pyrethroid insecticide is the development of resistance mechanisms, such as knockdown resistance or kdr. The objective of this study was to evaluate the presence of kdr mutations in a partial region of the VGSC gene in samples of wild-caught Aedes mosquitoes from different locations of the Metropolitan Region of Panama.
RESULTS
RESULTS
Based on the analysis of 194 sequences of the VGSC gene, two kdr mutations (Ile1011Met and Val1016Gly) were detected in a specimen of Ae. aegypti. The frequency of kdr mutations in the evaluated samples of Ae. aegypti was 0.01.
CONCLUSIONS
CONCLUSIONS
This study provides evidence for a low frequency of kdr mutations in Ae. aegypti populations in Panama. It is possible that these changes have no impact on vector control interventions. To our knowledge, we report, for the first time in America the Val1016Gly mutation documented in Asia. In general terms, this result is highly relevant to the Aedes Control Programme in Panama since it constitutes a feasible approach for the timely detection of resistance as well as for the development of strategies.
Identifiants
pubmed: 30691518
doi: 10.1186/s13071-019-3309-y
pii: 10.1186/s13071-019-3309-y
pmc: PMC6348643
doi:
Substances chimiques
Insecticides
0
Pyrethrins
0
Voltage-Gated Sodium Channels
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
62Subventions
Organisme : Sistema nacional de investigadores (SENACYT)
ID : level 1
Organisme : Panama Dengue Fever Research
ID : 20142019
Organisme : Science and Innovation Fund 2015 grant (UK)
ID : PA003
Organisme : Ministerio de Economía y Finanzas
ID : 2014-2019
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