Optical Properties of Corals Distort Variable Chlorophyll Fluorescence Measurements.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
15
10
2018
accepted:
16
01
2019
pubmed:
30
1
2019
medline:
19
6
2019
entrez:
30
1
2019
Statut:
ppublish
Résumé
Pulse-amplitude-modulated (PAM) fluorimetry is widely used in photobiological studies of corals, as it rapidly provides numerous photosynthetic parameters to assess coral ecophysiology. Coral optics studies have revealed the presence of light gradients in corals, which are strongly affected by light scattering in coral tissue and skeleton. We investigated whether coral optics affects variable chlorophyll (Chl) fluorescence measurements and derived photosynthetic parameters by developing planar hydrogel slabs with immobilized microalgae and with bulk optical properties similar to those of different types of corals. Our results show that PAM-based measurements of photosynthetic parameters differed substantially between hydrogels with different degrees of light scattering but identical microalgal density, yielding deviations in apparent maximal electron transport rates by a factor of 2. Furthermore, system settings such as the measuring light intensity affected
Identifiants
pubmed: 30692219
pii: pp.18.01275
doi: 10.1104/pp.18.01275
pmc: PMC6446749
doi:
Substances chimiques
Hydrogels
0
Chlorophyll
1406-65-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1608-1619Informations de copyright
© 2019 American Society of Plant Biologists. All Rights Reserved.
Références
Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8007-12
pubmed: 10393938
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10302-5
pubmed: 11607337
J Opt Soc Am A Opt Image Sci Vis. 2003 Apr;20(4):714-27
pubmed: 12683499
Methods Enzymol. 2005;397:166-99
pubmed: 16260291
Plant Physiol. 1992 Sep;100(1):367-73
pubmed: 16652970
J Biomed Opt. 2006 Jul-Aug;11(4):041102
pubmed: 16965130
Annu Rev Plant Biol. 2008;59:89-113
pubmed: 18444897
J Exp Biol. 2008 Oct;211(Pt 19):3059-66
pubmed: 18805804
Plant Cell Physiol. 2009 Apr;50(4):698-706
pubmed: 19282373
Biomed Opt Express. 2010 Aug 2;1(1):165-75
pubmed: 21170299
New Phytol. 2011 Jul;191(1):146-59
pubmed: 21418065
PLoS One. 2012;7(1):e30167
pubmed: 22253915
Front Microbiol. 2012 Aug 27;3:316
pubmed: 22969755
Appl Microbiol Biotechnol. 2013 Mar;97(5):1809-19
pubmed: 23321911
Plant Cell Environ. 2013 Oct;36(10):1755-70
pubmed: 23586649
PLoS One. 2013 Apr 22;8(4):e61492
pubmed: 23630594
Phys Med Biol. 2013 Jun 7;58(11):R37-61
pubmed: 23666068
Photosynth Res. 1996 Jan;47(1):103-9
pubmed: 24301712
Photosynth Res. 1994 Apr;40(1):67-74
pubmed: 24311215
Photosynth Res. 1993 Apr;36(1):65-72
pubmed: 24318799
J Exp Biol. 2014 Feb 15;217(Pt 4):489-98
pubmed: 24523498
Plant Physiol Biochem. 2014 Oct;83:159-67
pubmed: 25146689
Photosynth Res. 2015 Jan;123(1):77-92
pubmed: 25218266
PLoS One. 2014 Nov 12;9(11):e112809
pubmed: 25389753
New Phytol. 2015 Aug;207(3):559-69
pubmed: 25827160
ISME J. 2016 Mar;10(3):788-92
pubmed: 26241503
Photochem Photobiol. 2016 Mar;92(2):331-342
pubmed: 26715143
Front Microbiol. 2016 Feb 26;7:230
pubmed: 26955372
BMC Ecol. 2016 Mar 21;16:10
pubmed: 26996922
Sci Rep. 2016 May 26;6:26599
pubmed: 27225857
Front Plant Sci. 2016 Sep 21;7:1404
pubmed: 27708657
Front Microbiol. 2017 Jan 24;8:59
pubmed: 28174567
J R Soc Interface. 2017 Mar;14(128):
pubmed: 28250104
Oecologia. 1974 Dec;17(4):281-291
pubmed: 28308943
Plant Physiol. 2017 Oct;175(2):721-733
pubmed: 28821593
Plant Cell Physiol. 2017 Oct 1;58(10):1652-1660
pubmed: 29016964
Phys Med Biol. 1997 Jul;42(7):1415-26
pubmed: 9253049
Lasers Surg Med. 1997;21(2):166-78
pubmed: 9261794
Photochem Photobiol. 1998 Jan;67(1):23-32
pubmed: 9477762