Grafting as a Tool for Study of Phototropism in Phycomyces.

Phototropism / physiology Phycomyces / physiology
Complementation Fungi Grafting Phycomyces Sporangiophore

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 30 1 2019
pubmed: 30 1 2019
medline: 21 6 2019
Statut: ppublish

Résumé

Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.

Identifiants

DOI: 10.1007/978-1-4939-9015-3_16 PMID: 30694477
pubmed: 30694477
doi: 10.1007/978-1-4939-9015-3_16
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

199-206

Auteurs

Atsushi Miyazaki (A)

Department of Biological Sciences, Faculty of Science and Engineering, Ishinomaki Senshu University, Ishinomaki, Miyagi, Japan. miyazaki@isenshu-u.ac.jp.

Tamotsu Ootaki (T)

Tohoku University, Sendai, Japan.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques des plantes › Tropisme › Phototropisme › Grafting as a Tool for Study of Phototropism...
questionsmedicales.fr › Eucaryotes › Champignons › Champignons non classés › Mucorales › Phycomyces › Grafting as a Tool for Study of Phototropism...